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Side-chain-selective deuterium labeling by a simple bio-expression method enhances resolution and simplifies spectra in H-detected protein solid-state NMR
Side-chain-selective deuterium labeling by a simple bio-expression method enhances resolution and simplifies spectra in H-detected protein solid-state NMR
We present a novel approach for side-chain-selective deuteration of proteins to improve 1Hα spectral resolution and t...
Toward site-specific characterization of structural perturbations on glycosylated Fc using NMR at natural abundance
Toward site-specific characterization of structural perturbations on glycosylated Fc using NMR at natural abundance
Monoclonal antibodies (mAbs) are leading therapeutic agents due to their high specificity and limited side effects. Ensuri...
Incorporating the BEST methodology in experiments for measuring paramagnetic relaxation enhancements
Incorporating the BEST methodology in experiments for measuring paramagnetic relaxation enhancements
Band-selective excitation short-transient (BEST) sequences are widely used for protein NMR experiments that start with ami...
Single spin exact gradients for the optimization of complex pulses and pulse sequences
Single spin exact gradients for the optimization of complex pulses and pulse sequences
The efficient computer optimization of magnetic resonance pulses and pulse sequences involves the calculation of a problem...
F-NMR spectroscopy of fluorinated isoleucine analogues in a protein
F-NMR spectroscopy of fluorinated isoleucine analogues in a protein
The R3H domain of the human protein Sµbp-2 was produced with 5-fluoro-L-isoleucine (FIle) and 5,5-difluoro-L-isoleuci...
Web server DDfit: a new scheme to process PFG NMR diffusion data with improved precision
Web server DDfit: a new scheme to process PFG NMR diffusion data with improved precision
In this communication we describe a new scheme to process the data from stimulated echo protein diffusion experiments. For...
Conformation-selective detection of residues in solid proteins under magic-angle-spinning
Conformation-selective detection of residues in solid proteins under magic-angle-spinning
We demonstrate here a pulse sequence based on rotational-echo double resonance (REDOR) that can help distinguish resonance...
A fast and efficient strategy for the NMR assignment of Fab methyl groups
A fast and efficient strategy for the NMR assignment of Fab methyl groups
Owing to their high specificity and therapeutic effectiveness, monoclonal antibodies (mAbs) have rapidly become one of the...
M9 medium composition alters metabolism during recombinant expression
M9 medium composition alters metabolism during recombinant expression
M9 minimal media and its enhanced variants (M9 + and M9++) are widely used for recombinant protein expression ...
, an alternative source for isotopic enrichment of proteins expressed in insect cells for NMR investigations
, an alternative source for isotopic enrichment of proteins expressed in insect cells for NMR investigations
Isotopic enrichment of pharmacologically relevant protein targets is crucial for structural studies by nuclear magnetic re...
Optimus peak: automatic peak information extraction from 2D and 3D solution and solid state spectra using evolutionary algorithms
Optimus peak: automatic peak information extraction from 2D and 3D solution and solid state spectra using evolutionary algorithms
Peak information extraction is an important step for all types of protein NMR experiments, from structure determination to...
Super-Resolution solid-state NMR Spectroscopy
Super-Resolution solid-state NMR Spectroscopy
Solid-state NMR spectroscopy is often limited by low spectral resolution, a problem typically addressed using fast magic-a...
AMIGO - Guided assignment of C-methyl labelled proteins
AMIGO - Guided assignment of C-methyl labelled proteins
Over the last 20 years, the number of large proteins accessible to protein-NMR analysis has increased significantly due to...
Methyl-specific NMR of therapeutic antibodies: cost-effective isotopic labeling strategies in CHO cells for high-resolution structural characterization
Methyl-specific NMR of therapeutic antibodies: cost-effective isotopic labeling strategies in CHO cells for high-resolution structural characterization
Monoclonal antibodies (mAbs) are essential therapeutic agents whose efficacy depends critically on the integrity of their ...
A complete set of cross-correlated relaxation experiments for determining the protein backbone dihedral angles
A complete set of cross-correlated relaxation experiments for determining the protein backbone dihedral angles
The investigation of structural propensities of proteins is essential for understanding how they function at the molecular...
Quantifying protein-drug lifetimes in human cells by F NMR spectroscopy
Quantifying protein-drug lifetimes in human cells by F NMR spectroscopy
The cellular environment is a complex and crowded space, with organelles, compartments and multitudes of molecules engaged...
COLMARvista: an open source 2D and pseudo-3D NMR spectral processing, visualization, and analysis software in JavaScript
COLMARvista: an open source 2D and pseudo-3D NMR spectral processing, visualization, and analysis software in JavaScript
COLMARvista is presented as a new, highly versatile software for the easy and intuitive processing and visual inspection o...
Membrane protein structure determination from Paramagnetic Relaxation Enhancement and internuclear distance restraints
Membrane protein structure determination from Paramagnetic Relaxation Enhancement and internuclear distance restraints
Magic angle spinning nuclear magnetic resonance (MAS NMR) is well suited for the determination of protein structure. The k...
The SOFAST-HMBC-HMQC experiment for pairing geminal methyl groups in valine and leucine side-chains
The SOFAST-HMBC-HMQC experiment for pairing geminal methyl groups in valine and leucine side-chains
Methyl groups are essential probes for characterising interactions and dynamics in large proteins. HN-based triple-resonan...
Evaluating the use of lanthanide containing dendrimers for solvent paramagnetic relaxation enhancement
Evaluating the use of lanthanide containing dendrimers for solvent paramagnetic relaxation enhancement
Paramagnetic relaxation enhancement (PRE) is widely used in biomolecular NMR spectroscopy to obtain long-range distance an...
Estimating cross-relaxation rates between methyl and neighboring labile proton spins in high molecular weight proteins
Estimating cross-relaxation rates between methyl and neighboring labile proton spins in high molecular weight proteins
We show that water saturation leads to deleterious losses in sensitivity of methyl signals in selectively methyl-[13CH3]-l...
Extending the detectable time window of fast protein dynamics using H E-CPMG
Extending the detectable time window of fast protein dynamics using H E-CPMG
Recent advances in high power NMR relaxation dispersion experiments have significantly enhanced our ability to study fast ...
Investigating structural and dynamic changes in cellulose due to nanocrystallization
Investigating structural and dynamic changes in cellulose due to nanocrystallization
Cellulose nanocrystals (CNCs) is synthesized from alpha-cellulose by acid hydrolysis method, and formation of nanocrystall...
Divide-and-conquer strategy for NMR studies of the γ-clamp loader complex
Divide-and-conquer strategy for NMR studies of the γ-clamp loader complex
The E. coli γ-clamp loader is a 200 kDa pentameric AAA + ATPase comprised of γ, δ and ...
An optimized C single-quantum CPMG relaxation dispersion experiment for investigating microsecond-to-millisecond timescale dynamics in large proteins
An optimized C single-quantum CPMG relaxation dispersion experiment for investigating microsecond-to-millisecond timescale dynamics in large proteins
Biomolecular dynamics in the microsecond-to-millisecond (µs–ms) timescale are linked to various biological func...
Analyzing sub-millisecond timescale protein dynamics using eCPMG experiments
Analyzing sub-millisecond timescale protein dynamics using eCPMG experiments
Cellular functions require biomolecules to transition among various conformational sub-states in the energy landscape. A m...
Measuring long-range contacts in a fully protonated protein at 105 kHz magic angle spinning
Measuring long-range contacts in a fully protonated protein at 105 kHz magic angle spinning
The use of 1H detection, made possible by very fast magic-angle spinning (MAS), has revolutionized the field of biomolecul...
Sedimentation of large, soluble proteins up to 140 kDa for 1H-detected MAS NMR and 13C DNP NMR – practical aspects
Sedimentation of large, soluble proteins up to 140 kDa for 1H-detected MAS NMR and 13C DNP NMR – practical aspects
Solution NMR is typically applied to biological systems with molecular weights < 40 kDa wherea...
Micromolar fluoride contamination arising from glass NMR tubes and a simple solution for biomolecular applications
Micromolar fluoride contamination arising from glass NMR tubes and a simple solution for biomolecular applications
Fluorine (19F) NMR is emerging as an invaluable analytical technique in chemistry, biochemistry, structural biology, mater...
Deuterium spin relaxation of fractionally deuterated ribonuclease H using paired 475 and 950 MHz NMR spectrometers
Deuterium spin relaxation of fractionally deuterated ribonuclease H using paired 475 and 950 MHz NMR spectrometers
Deuterium (2H) spin relaxation of 13CH2D methyl groups has been widely applied to investigate picosecond-to-nanosecond con...
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