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A complete set of cross-correlated relaxation experiments for determining the protein backbone dihedral angles
A complete set of cross-correlated relaxation experiments for determining the protein backbone dihedral angles
The investigation of structural propensities of proteins is essential for understanding how they function at the molecular...
Quantifying protein-drug lifetimes in human cells by F NMR spectroscopy
Quantifying protein-drug lifetimes in human cells by F NMR spectroscopy
The cellular environment is a complex and crowded space, with organelles, compartments and multitudes of molecules engaged...
COLMARvista: an open source 2D and pseudo-3D NMR spectral processing, visualization, and analysis software in JavaScript
COLMARvista: an open source 2D and pseudo-3D NMR spectral processing, visualization, and analysis software in JavaScript
COLMARvista is presented as a new, highly versatile software for the easy and intuitive processing and visual inspection o...
Membrane protein structure determination from Paramagnetic Relaxation Enhancement and internuclear distance restraints
Membrane protein structure determination from Paramagnetic Relaxation Enhancement and internuclear distance restraints
Magic angle spinning nuclear magnetic resonance (MAS NMR) is well suited for the determination of protein structure. The k...
The SOFAST-HMBC-HMQC experiment for pairing geminal methyl groups in valine and leucine side-chains
The SOFAST-HMBC-HMQC experiment for pairing geminal methyl groups in valine and leucine side-chains
Methyl groups are essential probes for characterising interactions and dynamics in large proteins. HN-based triple-resonan...
Evaluating the use of lanthanide containing dendrimers for solvent paramagnetic relaxation enhancement
Evaluating the use of lanthanide containing dendrimers for solvent paramagnetic relaxation enhancement
Paramagnetic relaxation enhancement (PRE) is widely used in biomolecular NMR spectroscopy to obtain long-range distance an...
Estimating cross-relaxation rates between methyl and neighboring labile proton spins in high molecular weight proteins
Estimating cross-relaxation rates between methyl and neighboring labile proton spins in high molecular weight proteins
We show that water saturation leads to deleterious losses in sensitivity of methyl signals in selectively methyl-[13CH3]-l...
Extending the detectable time window of fast protein dynamics using H E-CPMG
Extending the detectable time window of fast protein dynamics using H E-CPMG
Recent advances in high power NMR relaxation dispersion experiments have significantly enhanced our ability to study fast ...
Investigating structural and dynamic changes in cellulose due to nanocrystallization
Investigating structural and dynamic changes in cellulose due to nanocrystallization
Cellulose nanocrystals (CNCs) is synthesized from alpha-cellulose by acid hydrolysis method, and formation of nanocrystall...
Divide-and-conquer strategy for NMR studies of the γ-clamp loader complex
Divide-and-conquer strategy for NMR studies of the γ-clamp loader complex
The E. coli γ-clamp loader is a 200 kDa pentameric AAA + ATPase comprised of γ, δ and ...
An optimized C single-quantum CPMG relaxation dispersion experiment for investigating microsecond-to-millisecond timescale dynamics in large proteins
An optimized C single-quantum CPMG relaxation dispersion experiment for investigating microsecond-to-millisecond timescale dynamics in large proteins
Biomolecular dynamics in the microsecond-to-millisecond (µs–ms) timescale are linked to various biological func...
Analyzing sub-millisecond timescale protein dynamics using eCPMG experiments
Analyzing sub-millisecond timescale protein dynamics using eCPMG experiments
Cellular functions require biomolecules to transition among various conformational sub-states in the energy landscape. A m...
Measuring long-range contacts in a fully protonated protein at 105 kHz magic angle spinning
Measuring long-range contacts in a fully protonated protein at 105 kHz magic angle spinning
The use of 1H detection, made possible by very fast magic-angle spinning (MAS), has revolutionized the field of biomolecul...
Sedimentation of large, soluble proteins up to 140 kDa for 1H-detected MAS NMR and 13C DNP NMR – practical aspects
Sedimentation of large, soluble proteins up to 140 kDa for 1H-detected MAS NMR and 13C DNP NMR – practical aspects
Solution NMR is typically applied to biological systems with molecular weights < 40 kDa wherea...
Micromolar fluoride contamination arising from glass NMR tubes and a simple solution for biomolecular applications
Micromolar fluoride contamination arising from glass NMR tubes and a simple solution for biomolecular applications
Fluorine (19F) NMR is emerging as an invaluable analytical technique in chemistry, biochemistry, structural biology, mater...
Deuterium spin relaxation of fractionally deuterated ribonuclease H using paired 475 and 950 MHz NMR spectrometers
Deuterium spin relaxation of fractionally deuterated ribonuclease H using paired 475 and 950 MHz NMR spectrometers
Deuterium (2H) spin relaxation of 13CH2D methyl groups has been widely applied to investigate picosecond-to-nanosecond con...
Fluorine-19 labeling of the tryptophan residues in the G protein-coupled receptor NK1R using the 5-fluoroindole precursor in Pichia pastoris expression
Fluorine-19 labeling of the tryptophan residues in the G protein-coupled receptor NK1R using the 5-fluoroindole precursor in Pichia pastoris expression
In NMR spectroscopy of biomolecular systems, the use of fluorine-19 probes benefits from a clean background and high sensi...
Towards cost-effective side-chain isotope labelling of proteins expressed in human cells
Towards cost-effective side-chain isotope labelling of proteins expressed in human cells
Side chain isotope labelling is a powerful tool to study protein structure and interactions by NMR spectroscopy. 1H,13C la...
Quantitative analysis of the slow exchange process by 19F NMR in the presence of scalar and dipolar couplings: applications to the ribose 2′-19F probe in nucleic acids
Quantitative analysis of the slow exchange process by 19F NMR in the presence of scalar and dipolar couplings: applications to the ribose 2′-19F probe in nucleic acids
Solution NMR spectroscopy is a particularly powerful technique for characterizing the functional dynamics of biomolecules,...
Transverse relaxation optimized spectroscopy of NH2 groups in glutamine and asparagine side chains of proteins
Transverse relaxation optimized spectroscopy of NH2 groups in glutamine and asparagine side chains of proteins
A transverse relaxation optimized spectroscopy (TROSY) approach is described for the optimal detection of NH2 groups in as...
Optimising in-cell NMR acquisition for nucleic acids
Optimising in-cell NMR acquisition for nucleic acids
Understanding the structure and function of nucleic acids in their native environment is crucial to structural biology and...
19F NMR relaxation of buried tryptophan side chains suggest anisotropic rotational diffusion of the protein RfaH
19F NMR relaxation of buried tryptophan side chains suggest anisotropic rotational diffusion of the protein RfaH
The recent application of 19F NMR in the study of biomolecular structure and dynamics has made it a potentially attractive...
Reducing experimental time through spin-lattice relaxation enhancement via dissolved oxygen
Reducing experimental time through spin-lattice relaxation enhancement via dissolved oxygen
Large proteins and dilute spin systems within a deuterated background are often characterized by long proton (1H) spin-lat...
Local structure propensities in disordered proteins from cross-correlated NMR spin relaxation
Local structure propensities in disordered proteins from cross-correlated NMR spin relaxation
Structurally diverse ensembles of intrinsically disordered proteins or regions are difficult to determine, because experim...
Pitfalls in measurements of R1 relaxation rates of protein backbone 15N nuclei
Pitfalls in measurements of R1 relaxation rates of protein backbone 15N nuclei
The dynamics of the backbone and side-chains of protein are routinely studied by interpreting experimentally determined 15...
Alpha-helices as alignment reporters in residual dipolar coupling analysis of proteins
Alpha-helices as alignment reporters in residual dipolar coupling analysis of proteins
Inclusion of residual dipolar couplings (RDCs) during the early rounds of protein structure determination requires use of ...
Evaluation of TOCSY mixing for sensitivity-enhancement in solid-state NMR and application of 4D experiments for side-chain assignments of the full-length 30 kDa membrane protein GlpG
Evaluation of TOCSY mixing for sensitivity-enhancement in solid-state NMR and application of 4D experiments for side-chain assignments of the full-length 30 kDa membrane protein GlpG
Chemical shift assignments of large membrane proteins by solid-state NMR experiments are challenging. Recent advancements ...
Solid state NMR spectral editing of histidine, arginine and lysine using Hadamard encoding
Solid state NMR spectral editing of histidine, arginine and lysine using Hadamard encoding
The NMR signals from protein sidechains are rich in information about intra- and inter-molecular interactions, but their d...
15N-detected TROSY for 1H-15N heteronuclear correlation to study intrinsically disordered proteins: strategies to increase spectral quality
15N-detected TROSY for 1H-15N heteronuclear correlation to study intrinsically disordered proteins: strategies to increase spectral quality
Intrinsically disordered proteins and protein regions are central to many biological processes but difficult to characteri...
Using temperature coefficients to support resonance assignment of intrinsically disordered proteins
Using temperature coefficients to support resonance assignment of intrinsically disordered proteins
The resonance assignment of large intrinsically disordered proteins (IDPs) is difficult due to the low dispersion of chemi...
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