A transgene-free, human peri-gastrulation embryo model presents trilaminar embryonic disc-, amnion- and yolk sac-like structures

Tam, P. P. L. & Loebel, D. A. F. Gene function in mouse embryogenesis: get set for gastrulation. Nat. Rev. Genet. 8, 368–381 (2007).

Article  CAS  PubMed  Google Scholar 

Arnold, S. J. & Robertson, E. J. Making a commitment: cell lineage allocation and axis patterning in the early mouse embryo. Nat. Rev. Mol. Cell Biol. 10, 91–103 (2009).

Article  CAS  PubMed  Google Scholar 

Solnica-Krezel, L. in Gastrulation: From Embryonic Pattern to Form Vol. 136, xvii–xxiv (Academic, 2020).

Sheng, G. J., Arias, A. M. & Sutherland, A. The primitive streak and cellular principles of building an amniote body through gastrulation. Science 374, eabg1727 (2021).

Article  CAS  Google Scholar 

O’Rahilly, R. & Muller, F. Developmental stages in human embryos: revised and new measurements. Cells Tissues Organs 192, 73–84 (2010).

Article  PubMed  Google Scholar 

Shahbazi, M. N., Siggia, E. D. & Zernicka-Goetz, M. Self-organization of stem cells into embryos: a window on early mammalian development. Science 364, 948–951 (2019).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Fu, J., Warmflash, A. & Lutolf, M. P. Stem-cell-based embryo models for fundamental research and translation. Nat. Mater. 20, 132–144 (2021).

Article  PubMed  Google Scholar 

Rossant, J. & Tam, P. P. L. Opportunities and challenges with stem cell-based embryo models. Stem Cell Rep. 16, 1031–1038 (2021).

Article  Google Scholar 

Warmflash, A., Sorre, B., Etoc, F., Siggia, E. D. & Brivanlou, A. H. A method to recapitulate early embryonic spatial patterning in human embryonic stem cells. Nat. Methods 11, 847–854 (2014).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Shao, Y. et al. A pluripotent stem cell-based model for post-implantation human amniotic sac development. Nat. Commun. 8, 208 (2017).

Article  PubMed  PubMed Central  Google Scholar 

Simunovic, M. et al. A 3D model of a human epiblast reveals BMP4-driven symmetry breaking. Nat. Cell Biol. 21, 900–910 (2019).

Article  CAS  PubMed  Google Scholar 

Ai, Z. et al. Dissecting peri-implantation development using cultured human embryos and embryo-like assembloids. Cell Res. 33, 661–678 (2023).

Article  PubMed  PubMed Central  Google Scholar 

Karvas, R. M. et al. 3D-cultured blastoids model human embryogenesis from pre-implantation to early gastrulation stages. Cell Stem Cell 30, 1148–1165 (2023).

Article  CAS  PubMed  Google Scholar 

Liu, L. et al. Modeling post-implantation stages of human development into early organogenesis with stem-cell-derived peri-gastruloids. Cell 186, 3776–3792 (2023).

Article  CAS  PubMed  Google Scholar 

Oldak, B. et al. Complete human day 14 post-implantation embryo models from naive ES cells. Nature 622, 562–573 (2023).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Pedroza, M. et al. Self-patterning of human stem cells into post-implantation lineages. Nature 622, 574–583 (2023).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Weatherbee, B. A. T. et al. Pluripotent stem cell-derived model of the post-implantation human embryo. Nature 622, 584–593 (2023).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Hislop, J. et al. Modeling post-implantation human development to yolk sac blood emergence. Nature 626, 367–376 (2023).

Okubo, T. et al. Hypoblast from human pluripotent stem cells regulates epiblast development. Nature 626, 357–366 (2024).

Article  CAS  PubMed  Google Scholar 

Yang, R. et al. Amnion signals are essential for mesoderm formation in primates. Nat. Commun. 12, 5126 (2021).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Mackinlay, K. M. L. et al. An in vitro stem cell model of human epiblast and yolk sac interaction. eLife 10, e63930 (2021).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Chen, L. et al. The nuclear receptor HNF4 drives a brush border gene program conserved across murine intestine, kidney, and embryonic yolk sac. Nat. Commun. 12, 2886 (2021).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Ross, C. & Boroviak, T. E. Origin and function of the yolk sac in primate embryogenesis. Nat. Commun. 11, 3760 (2020).

Article  PubMed  PubMed Central  Google Scholar 

Brennan, J. et al. Nodal signalling in the epiblast patterns the early mouse embryo. Nature 411, 965–969 (2001).

Article  CAS  PubMed  Google Scholar 

Rivera-Pérez, J. A. & Magnuson, T. Primitive streak formation in mice is preceded by localized activation of Brachyury and Wnt3. Dev. Biol. 288, 363–371 (2005).

Article  PubMed  Google Scholar 

Ben-Haim, N. et al. The nodal precursor acting via activin receptors induces mesoderm by maintaining a source of its convertases and BMP4. Dev. Cell 11, 313–323 (2006).

Article  CAS  PubMed  Google Scholar 

Chhabra, S., Liu, L., Goh, R., Kong, X. & Warmflash, A. Dissecting the dynamics of signaling events in the BMP, WNT, and NODAL cascade during self-organized fate patterning in human gastruloids. PLoS Biol. 17, e3000498 (2019).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Muncie, J. M. et al. Mechanical tension promotes formation of gastrulation-like nodes and patterns mesoderm specification in human embryonic stem cells. Dev. Cell 55, 679–694 (2020).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Tesar, P. J. et al. New cell lines from mouse epiblast share defining features with human embryonic stem cells. Nature 448, 196–199 (2007).

Article  CAS  PubMed  Google Scholar 

O’Leary, T. et al. Tracking the progression of the human inner cell mass during embryonic stem cell derivation. Nat. Biotechnol. 30, 278–282 (2012).

Article  PubMed  Google Scholar 

Zheng, Y. et al. Controlled modelling of human epiblast and amnion development using stem cells. Nature 573, 421–425 (2019).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Sun, S. et al. A transgene-free, human peri-gastrulation embryo model with trilaminar embryonic disc-, amnion- and yolk sac-like structures. protocol.io https://doi.org/10.17504/protocols.io.rm7vzem34vx1/v1 (2026).

Karzbrun, E. et al. Human neural tube morphogenesis in vitro by geometric constraints. Nature 599, 268–272 (2021).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Xue, X. et al. Mechanics-guided embryonic patterning of neuroectoderm tissue from human pluripotent stem cells. Nat. Mater. 17, 633–641 (2018).

Article  CAS  PubMed  PubMed C

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