Arai S, Sakakibara S, Mareschal R, Ooi T, Zinn M, Matsumoto K (2020) Biosynthesis of random-homo block copolymer poly[glycolate-ran-3-hydroxybutyrate (3HB)]-b-poly(3HB) using sequence-regulating chimeric polyhydroxyalkanoate synthase in Escherichia coli. Front Bioeng Biotechnol 8:612991

PubMed  PubMed Central  Google Scholar 

Chai Y, Kolter R, Losick R (2009) A widely conserved gene cluster required for lactate utilization in Bacillus subtilis and its involvement in biofilm formation. J Bacteriol 191:2423–2430

CAS  PubMed  PubMed Central  Google Scholar 

Dennis D, McCoy M, Stangl A, Valentin HE, Wu Z (1998) Formation of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) by PHA synthase from Ralstonia eutropha. J Biotechnol 64:177–186

CAS  PubMed  Google Scholar 

Fukui T, Doi Y (1997) Cloning and analysis of the poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) biosynthesis genes of Aeromonas caviae. J Bacteriol 179:4821–4830

CAS  PubMed  PubMed Central  Google Scholar 

Fukui T, Mukoyama M, Orita I, Nakamura S (2014) Enhancement of glycerol utilization ability of Ralstonia eutropha H16 for production of polyhydroxyalkanoates. Appl Microbiol Biotechnol 98:7759–7768

Google Scholar 

Fukui T, Ohsawa K, Mifune J, Orita I, Nakamura S (2011) Evaluation of promoters for gene expression in polyhydroxyalkanoate-producing Cupriavidus necator H16. Appl Microbiol Biotechnol 89:1527–1536

CAS  PubMed  Google Scholar 

Guzik M, Witko T, Steinbüchel A, Wojnarowska M, Sołtysik M, Wawak S (2020) What has been trending in the research of polyhydroxyalkanoates? A systematic review. Front Bioeng Biotechnol 8:959

PubMed  PubMed Central  Google Scholar 

Insomphun C, Mifune J, Orita I, Numata K, Nakamura S, Fukui T (2014) Modification of β-oxidation pathway in Ralstonia eutropha for production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from soybean oil. J Biosci Bioeng 117:184–190

CAS  PubMed  Google Scholar 

Insomphun C, Xie H, Mifune J, Kawashima Y, Orita I, Nakamura S, Fukui T (2015) Improved artificial pathway for biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) with high C6-monomer composition from fructose in Ralstonia eutropha. Metab Eng 27:38–45

CAS  PubMed  Google Scholar 

Kageyama Y, Tomita H, Isono T, Satoh T, Matsumoto K (2021) Artificial polyhydroxyalkanoate poly[2-hydroxybutyrate-block-3-hydroxybutyrate] elastomer-like material. Sci Rep 11:2244

Google Scholar 

Kato M, Bao HJ, Kang CK, Fukui T, Doi Y (1996) Production of a novel copolymers of 3-hydoroxybutyric acid and medium-chain length 3-hydoroxyalkanoic acid by Psudomonas sp. 61-3 from sugars. Appl Microbiol Biotechnol 45:363–370

CAS  Google Scholar 

Kawashima Y, Orita I, Nakamura S, Fukui T (2015) Compositional regulation of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) by replacement of granule-associated protein in Ralstonia eutropha. Microb Cell Fact 14:187

PubMed  PubMed Central  Google Scholar 

Kim J, Darley D, Selmer T, Buckel W (2006) Characterization of (R)-2-hydroxyisocaproate dehydrogenase and a family III coenzyme A transferase involved in reduction of l-leucine to isocaproate by Clostridium difficile. Appl Environ Microbiol 72:6062–6069

CAS  PubMed  PubMed Central  Google Scholar 

Kovach ME, Elzer PH, Hill DS, Robertson GT, Farris MA, Roop RM 2nd, Peterson KM (1995) Four new derivatives of the broad-host-range cloning vector pBBR1MCS, carrying different antibiotic-resistance cassettes. Gene 166:175–176

CAS  PubMed  Google Scholar 

Kumar N, Ravikumar MN, Domb AJ (2001) Biodegradable block copolymers. Adv Drug Deliv Rev 53:23–44

CAS  PubMed  Google Scholar 

Li Y, Wei H, Wang T, Xu Q, Zhang C, Fan X, Ma Q, Chen N, Xie X (2017) Current status on metabolic engineering for the production of l-aspartate family amino acids and derivatives. Bioresour Technol 245(Pt B):1588–1602

CAS  PubMed  Google Scholar 

Lu J, Brigham CJ, Plassmeier JK, Sinskey AJ (2015) Characterization and modification of enzymes in the 2-ketoisovalerate biosynthesis pathway of Ralstonia eutropha H16. Appl Microbiol Biotechnol 99:761–774

CAS  PubMed  Google Scholar 

Matsumoto K, Hori C, Fujii R, Takaya M, Ooba T, Ooi T, Isono T, Satoh T, Taguchi S (2018) Dynamic changes of intracellular monomer levels regulate block sequence of polyhydroxyalkanoates in engineered Escherichia coli. Biomacromolecules 19:662–671

CAS  PubMed  Google Scholar 

Matsumoto K, Takase K, Yamamoto Y, Doi Y, Taguchi S (2009) Chimeric enzyme composed of polyhydroxyalkanoate (PHA) synthases from Ralstonia eutropha and Aeromonas caviae enhances production of PHAs in recombinant Escherichia coli. Biomacromolecules 10:682–685

CAS  PubMed  Google Scholar 

Matsusaki H, Manji S, Taguchi K, Kato M, Fukui T, Doi Y (1998) Cloning and molecular analysis of the poly(3-hydroxybutyrate) and poly(3-hydroxybutyrate-co-3-hydroxyalkanoate) biosynthesis genes in Pseudomonas sp. strain 61-3. J Bacteriol 180:6459–6467

CAS  PubMed  PubMed Central  Google Scholar 

Mierzati M, Mizuno S, Tsuge T (2020) Biosynthesis and characterization of poly(3-hydroxybutyrate-co-2-hydroxyalkanoate) with different comonomer fractions. Polym Degrad Stab 178:109193

CAS  Google Scholar 

Mifune J, Nakamura S, Fukui T (2010) Engineering of pha operon on Cupriavidus necator chromosome for efficient biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from vegetable oil. Polym Degrad Stab 95:1305–1312

CAS  Google Scholar 

Miyahara Y, Hiroe A, Sato S, Tsuge T, Taguchi S (2020) Microbial polyhydroxyalkanoates (PHAs): from synthetic biology to industrialization. In: Rehm B, Moradali MF (eds) Biopolymers for biomedical and biotechnological applications, 1st edn. Wiley-VCH, Germany, pp 231–264

Google Scholar 

Mizuno S, Enda Y, Saika A, Hiroe A, Tsuge T (2018) Biosynthesis of polyhydroxyalkanoates containing 2-hydroxy-4-methylvalerate and 2-hydroxy-3-phenylpropionate units from a related or unrelated carbon source. J Biosci Bioeng 125:295–300

CAS  PubMed  Google Scholar 

Narancic T, Verstichel S, Reddy Chaganti S, Morales-Gamez L, Kenny ST, De Wilde B, Babu Padamati R, O’Connor KE (2018) Biodegradable plastic blends create new possibilities for end-of-life management of plastics but they are not a panacea for plastic pollution. Environ Sci Technol 52:10441–10452

CAS  PubMed  Google Scholar 

Nduko JM, Matsumoto K, Ooi T, Taguchi S (2013) Effectiveness of xylose utilization for high yield production of lactate-enriched P(lactate-co-3-hydroxybutyrate) using a lactate-overproducing strain of Escherichia coli and an evolved lactate-polymerizing enzyme. Metab Eng 15:159–166

CAS  PubMed  Google Scholar 

Neoh SZ, Chek MF, Tan HT, Pastén JAL, Nandakumar A, Hakoshima T, Sudesh K (2022) Polyhydroxyalkanoate synthase (PhaC): the key enzyme for biopolyester synthesis. Curr Res Biotechnol 4:87–101

Google Scholar 

Orita I, Iwazawa R, Nakamura S, Fukui T (2012) Identification of mutation points in Cupriavidus necator NCIMB 11599 and genetic reconstitution of glucose-utilization ability in wild strain H16 for polyhydroxyalkanoate production. J Biosci Bioeng 113:63–69

CAS  PubMed  Google Scholar 

Park SJ, Jang YA, Lee H, Park AR, Yang JE, Shin J, Oh YH, Song BK, Jegal J, Lee SH, Lee SY (2013) Metabolic engineering of Ralstonia eutropha for the biosynthesis of 2-hydroxyacid-containing polyhydroxyalkanoates. Metab Eng 20:20–28

CAS  PubMed  Google Scholar 

Pohlmann A, Fricke WF, Reinecke F, Kusian B, Liesegang H, Cramm R, Eitinger T, Ewering C, Pötter M, Schwartz E, Strittmatter A, Voss I, Gottschalk G, Steinbüchel A, Friedrich B, Bowien B (2006) Genome sequence of the bioplastic-producing “Knallgas” bacterium Ralstonia eutropha H16. Nat Biotechnol 24:1257–1262

PubMed  Google Scholar 

Pötter M, Madkour MH, Mayer F, Steinbüchel A (2002) Regulation of phasin expression and polyhydroxyalkanoate (PHA) granule formation in Ralstonia eutropha H16. Microbiology 148:2413–2426

PubMed  Google Scholar 

Satoh K, Kawakami T, Isobe N, Pasquier L, Tomita H, Zinn M, Matsumoto K (2022) Versatile aliphatic polyester biosynthesis system for producing random and block copolymers composed of 2-, 3-, 4-, 5-, and 6-hydroxyalkanoates using the sequence-regulating polyhydroxyalkanoate synthase PhaCAR. Microb Cell Fact 21:84

CAS  PubMed  PubMed Central  Google Scholar 

Schäfer A, Tauch A, Jäger W, Kalinowski J, Thierbach G, Pühler A (1994) Small mobilizable multi-purpose cloning vectors derived from the Escherichia coli plasmids pK18 and pK19: selection of defined deletions in the chromosome of Corynebacterium glutamicum. Gene 145:69–73

PubMed  Google Scholar 

Shozui F, Matsumoto K, Motohashi R, Sun J, Satoh T, Kakuchi T, Taguchi S (2011) Biosynthesis of a lactate (LA)-based polyester with a 96 mol% LA fraction and its application to stereocomplex formation. Polym Degrad Stab 96:499–504

CAS  Google Scholar 

Shozui F, Matsumoto K, Nakai T, Yamada M, Taguchi S (2010) Biosynthesis of novel terpolymers poly(lactate-co-3-hydroxybutyrate-co-3-hydroxyvalerate)s in lactate-overproducing mutant Escherichia coli JW0885 by feeding propionate as a precursor of 3-hydroxyvalerate. Appl Microbiol Biotechnol 85:949–954

CAS  PubMed  Google Scholar 

Simon R, Priefer U, Pühler A (1983) A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in gram negative bacteria. Bio/Technology 1:784–791

Stark MJ (1987) Multicopy expression vectors carrying the lac repressor gene for regulated high-level expression of genes in Escherichia coli. Gene 51:255–267

CAS  PubMed  Google Scholar 

Sudo M, Hori C, Ooi T, Mizuno S, Tsuge T, Matsumoto K (2020) Synergy of valine and threonine supplementation on poly(2-hydroxybutyrate-block-3-hydroxybutyrate) synthesis in engineered Escherichia coli expressing chimeric polyhydroxyalkanoate synthase. J Biosci Bioeng 129:302–306

CAS  PubMed  Google Scholar 

Taguchi S, Matsumoto K (2021) Evolution of polyhydroxyalkanoate synthesizing systems toward a sustainable plastic industry. Polym J 53:67–79

CAS  Google Scholar 

Taguchi S, Yamada M, Matsumoto K, Tajima K, Satoh Y, Munekata M, Ohno K, Kohda K, Shimamura T, Kambe H, Obata S (2008) A microbial factory for lactate-based polyesters using a lactate-polymerizing enzyme. Proc Natl Acad Sci USA 105:17323–17327

CAS  PubMed  PubMed Central  Google Scholar 

Volodina E, Schürmann M, Lindenkamp N, Steinbüchel A (2014) Characterization of propionate CoA-transferase from Ralstonia eutropha H16. Appl Microbiol Biotechnol 98:3579–3589

CAS  PubMed  Google Scholar 

Waring RH, Harris RM, Mitchell SC (2018) Plastic contamination of the food chain: a threat to human health? Maturitas 115:64–68

CAS  PubMed  Google Scholar 

Zhang M, Kurita S, Orita I, Nakamura S, Fukui T (2019) Modification of acetoacetyl-CoA reduction step in Ralstonia eutropha for biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from structurally unrelated compounds. Microb Cell Fact 18:147

PubMed  PubMed Central  Google Scholar