Bonanno JA. Identity and regulation of ion transport mechanisms in the corneal endothelium. Prog Retin Eye Res. 2003;22:69–94.

Article  CAS  PubMed  Google Scholar 

Joyce NC. Proliferative capacity of the corneal endothelium. Prog Retin Eye Res. 2003;22:359–89.

Article  CAS  PubMed  Google Scholar 

Gain P, Jullienne R, He Z, Aldossary M, Acquart S, Cognasse F, et al. Global survey of corneal transplantation and eye banking. JAMA Ophthalmol. 2016;134:167–73.

Article  PubMed  Google Scholar 

Armitage WJ, Dick AD, Bourne WM. Predicting endothelial cell loss and long-term corneal graft survival. Investig Ophthalmol Vis Sci. 2003;44:3326–31.

Article  Google Scholar 

Ziakas N, Chranioti A, Tzamalis A, Giannoulis D, Kapantais D, Tsatsos M, et al. Descemet’s stripping automated endothelial keratoplasty using donor tissue from an 83-day-old infant. J Curr Ophthalmol. 2022;34:257–9.

Article  PubMed  PubMed Central  Google Scholar 

Rafat M, Jabbarvand M, Sharma N, Xeroudaki M, Tabe S, Omrani R, et al. Bioengineered corneal tissue for minimally invasive vision restoration in advanced keratoconus in two clinical cohorts. Nat Biotechnol. 2023;41:70–81.

Article  CAS  PubMed  Google Scholar 

Anshu A, Price MO, Price FW Jr. Risk of corneal transplant rejection significantly reduced with Descemet’s membrane endothelial keratoplasty. Ophthalmology. 2012;119:536–40.

Article  PubMed  Google Scholar 

Crapo PM, Gilbert TW, Badylak SF. An overview of tissue and whole organ decellularization processes. Biomaterials. 2011;32:3233–43.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Chen Z, You J, Liu X, Cooper S, Hodge C, Sutton G, et al. Biomaterials for corneal bioengineering. Biomed Mater. 2018;13:032002.

Article  PubMed  Google Scholar 

Gonzalez-Andrades M, de la Cruz CJ, Ionescu AM, Campos A, Del Mar PM, Alaminos M. Generation of bioengineered corneas with decellularized xenografts and human keratocytes. Investig Ophthalmol Vis Sci. 2011;52:215–22.

Article  CAS  Google Scholar 

Gonzalez-Andrades M, Carriel V, Rivera-Izquierdo M, Garzon I, Gonzalez-Andrades E, Medialdea S, et al. Effects of detergent-based protocols on decellularization of corneas with sclerocorneal limbus. Evaluation of regional differences. Transl Vis Sci Technol. 2015;4:13.

Article  PubMed  PubMed Central  Google Scholar 

Hashimoto Y, Funamoto S, Sasaki S, Negishi J, Honda T, Hattori S, et al. Corneal regeneration by deep anterior lamellar keratoplasty (DALK) using decellularized corneal matrix. PLoS ONE. 2015;10:e0131989.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Lee W, Miyagawa Y, Long C, Cooper DK, Hara H. A comparison of three methods of decellularization of pig corneas to reduce immunogenicity. Int J Ophthalmol. 2014;7:587–93.

Article  PubMed  PubMed Central  Google Scholar 

Bhattacharya R, Saha S, Kostina O, Muravnik L, Mitra A. Replacing critical point drying with a low-cost chemical drying provides comparable surface image quality of glandular trichomes from leaves of Millingtonia hortensis L. f. in scanning electron micrograph. Appl Microsc. 2020;50:15.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Djulbegovic MB, Antonietti M, Taylor Gonzalez DJ, Mattes R, Kim C, Uversky VN, et al. Comparative analysis of the intrinsic disorder within the layers of the human cornea. Cornea. 2025;44:234–49.

Article  PubMed  Google Scholar 

Sagnial T, Ninotta S, Goin P, Aouimeur I, Parveau L, Poinard S, et al. Optimized laboratory techniques for assessing the quality of pre-stripped DMEK grafts. Sci Rep. 2025;15:7527.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Nakagawa T, Honda T, Yuasa T, Nishiuchi G, Sato M, Tokunaga A, et al. The TCF4 gene regulates apoptosis of corneal endothelial cells in Fuchs endothelial corneal dystrophy. Investig Ophthalmol Vis Sci. 2025;66:16.

Article  CAS  Google Scholar 

Wu KY, Belaiche M, Wen Y, Choulakian MY, Tran SD. Advancements in polymer biomaterials as scaffolds for corneal endothelium tissue engineering. Polymers (Basel). 2024;16:2882.

Article  CAS  PubMed  Google Scholar 

Rowe-Rendleman C, Eveleth D, Goldberg JL, Jurkunas U, Okumura N, Dawson D, et al. Development of anterior segment focused biologic therapies to regenerate corneal tissue for the treatment of disease: drug development experience. J Ocul Pharmacol Ther. 2023;39:551–62.

Article  CAS  PubMed  Google Scholar 

Kitazawa K, Sotozono C, Kinoshita S. Current advancements in corneal cell-based therapy. Asia Pac J Ophthalmol (Phila). 2022;11:335–45.

Article  PubMed  Google Scholar 

Hatou S, Shimmura S. Advances in corneal regenerative medicine with iPS cells. Jpn J Ophthalmol. 2023;67:541–5.

Article  PubMed  Google Scholar 

Li Y, Wang Z. Biomaterials for corneal regeneration. Adv Sci (Weinh). 2025;12:e2408021.

Article  CAS  PubMed  Google Scholar 

Hashimoto Y, Negishi J, Funamoto S, Kimura T, Kobayashi H, Oshika T, et al. Preparation, physico-biochemical characterization, and proteomic analysis of highly transparent corneal extracellular matrices for lamellar keratoplasty and tissue-engineered cornea construction. Mater Today Bio. 2024;28:101241.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Polisetti N, Schmid A, Schlotzer-Schrehardt U, Maier P, Lang SJ, Steinberg T, et al. A decellularized human corneal scaffold for anterior corneal surface reconstruction. Sci Rep. 2021;11:2992.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Li H, Zhao L, Wang F, Wang H, Dong M, Liu T, et al. Natural cross-linker-stabilized acellular porcine corneal stroma for lamellar keratoplasty. Acta Biomater. 2020;114:270–84.

Article  CAS  PubMed  Google Scholar 

Kim H, Jang J, Park J, Lee KP, Lee S, Lee DM, et al. Shear-induced alignment of collagen fibrils using 3D cell printing for corneal stroma tissue engineering. Biofabrication. 2019;11:035017.

Article  CAS  PubMed  Google Scholar 

Gupta S, Buyank F, Sinha NR, Grant DG, Sinha PR, Iozzo RV, et al. Decorin regulates collagen fibrillogenesis during corneal wound healing in mouse in vivo. Exp Eye Res. 2022;216:108933.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Gaetani R, Aude S, DeMaddalena LL, Strassle H, Dzieciatkowska M, Wortham M, et al. Evaluation of different decellularization protocols on the generation of pancreas-derived hydrogels. Tissue Eng Part C Methods. 2018;24:697–708.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Pereira AR, Trivanovic D, Stahlhut P, Rudert M, Groll J, Herrmann M. Preservation of the naive features of mesenchymal stromal cells In vitro: comparison of cell- and bone-derived decellularized extracellular matrix. J Tissue Eng. 2022;13:20417314221074452.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Hoshiba T, Yunoki S. Comparison of decellularization protocols for cultured cell-derived extracellular matrix—effects on decellularization efficacy, extracellular matrix retention, and cell functions. J Biomed Mater Res B Appl Biomater. 2023;111:85–94.

Article  CAS  PubMed  Google Scholar 

Espana EM, Birk DE. Composition, structure and function of the corneal stroma. Exp Eye Res. 2020;198:108137.

Article