Enhancing Long-Term Survival and Self-Renewal of Primary Hepatocytes via Rapid Spheroid Formation Using Rocker System through Co-Culturing with HUVEC Over-Expressing RSPO1

Younossi ZM, Koenig AB, Abdelatif D, Fazel Y, Henry L, Wymer M. Global epidemiology of nonalcoholic fatty liver disease-meta-analytic assessment of prevalence, incidence, and outcomes. Hepatology. 2016;64:73–84.

Article  Google Scholar 

de Rave S, Tilanus HW, van der Linden J, de Man RA, van der Berg B, Hop WC, et al. The importance of orthotopic liver transplantation in acute hepatic failure. Transplant Int. 2002;15:29–33.

Article  Google Scholar 

Farmer DG, Anselmo DM, Ghobrial RM, Yersiz H, McDiarmid SV, Cao C, et al. Liver transplantation for fulminant hepatic failure: experience with more than 200 patients over a 17-year period. Ann Surg. 2003;237:666–75.

Article  Google Scholar 

Kumar R, Anand U, Priyadarshi RN. Liver transplantation in acute liver failure: dilemmas and challenges. World J Transpl. 2021;11:187–202.

Article  Google Scholar 

He YT, Qi YN, Zhang BQ, Li JB, Bao J. Bioartificial liver support systems for acute liver failure: a systematic review and meta-analysis of the clinical and preclinical literature. World J Gastroenterol. 2019;25:3634–48.

Article  Google Scholar 

van de Kerkhove MP, Hoekstra R, Chamuleau RA, van Gulik TM. Clinical application of bioartificial liver support systems. Ann Surg. 2004;240:216–30.

Article  Google Scholar 

Weng J, Han X, Zeng F, Zhang Y, Feng L, Cai L, et al. Fiber scaffold bioartificial liver therapy relieves acute liver failure and extrahepatic organ injury in pigs. Theranostics. 2021;11:7620–39.

Article  CAS  Google Scholar 

Demetriou AA, Brown RS Jr, Busuttil RW, Fair J, McGuire BM, Rosenthal P, et al. Prospective, randomized, multicenter, controlled trial of a bioartificial liver in treating acute liver failure. Ann Surg. 2004;239:660–7.

Article  Google Scholar 

Sakiyama R, Blau BJ, Miki T. Clinical translation of bioartificial liver support systems with human pluripotent stem cell-derived hepatic cells. World J Gastroenterol. 2017;23:1974–9.

Article  CAS  Google Scholar 

Kumar A, Tripathi A, Jain S. Extracorporeal bioartificial liver for treating acute liver diseases. J Extra Corpor Technol. 2011;43:195–206.

Google Scholar 

Han B, Shi XL, Zhang Y, Gu ZZ, Yuan XW, Ren HZ, et al. No transmission of porcine endogenous retrovirus in an acute liver failure model treated by a novel hybrid bioartificial liver containing porcine hepatocytes. Hepatobiliary Pancreat Dis Int HBPD INT. 2015;14:492–501.

Article  CAS  Google Scholar 

Nyberg SL, Remmel RP, Mann HJ, Peshwa MV, Hu WS, Cerra FB. Primary hepatocytes outperform Hep G2 cells as the source of biotransformation functions in a bioartificial liver. Ann Surg. 1994;220:59–67.

CAS  Google Scholar 

Michalopoulos GK, DeFrances MC. Liver regeneration. Science (New York, NY). 1997;276:60–6.

Article  CAS  Google Scholar 

Zeng Q, Chen S, You Z, Yang F, Carey TE, Saims D, et al. Hepatocyte growth factor inhibits anoikis in head and neck squamous cell carcinoma cells by activation of ERK and Akt signaling independent of NFkappa B. J Biol Chem. 2002;277:25203–8.

Article  CAS  Google Scholar 

Chen H, Ma J, Liu J, Dou L, Shen T, Zuo H, et al. Lysophosphatidylcholine disrupts cell adhesion and induces anoikis in hepatocytes. FEBS Lett. 2022;596:510–25.

Article  CAS  Google Scholar 

Bachmann A, Moll M, Gottwald E, Nies C, Zantl R, Wagner H, et al. 3D cultivation techniques for primary human hepatocytes. Microarrays (Basel). 2015;4:64–83.

Article  CAS  PubMed  Google Scholar 

Mizoi K, Arakawa H, Yano K, Koyama S, Kojima H, Ogihara T. Utility of three-dimensional cultures of primary human hepatocytes (spheroids) as pharmacokinetic models. Biomedicines. 2020;8:374.

Article  PubMed  PubMed Central  Google Scholar 

Godoy P, Hewitt NJ, Albrecht U, Andersen ME, Ansari N, Bhattacharya S, et al. Recent advances in 2D and 3D in vitro systems using primary hepatocytes, alternative hepatocyte sources and non-parenchymal liver cells and their use in investigating mechanisms of hepatotoxicity, cell signaling and ADME. Arch Toxicol. 2013;87:1315–530.

Article  CAS  Google Scholar 

Liu M, Yang J, Hu W, Zhang S, Wang Y. Superior performance of co-cultured mesenchymal stem cells and hepatocytes in poly(lactic acid-glycolic acid) scaffolds for the treatment of acute liver failure. Biomed Mater. 2016;11:015008.

Article  CAS  Google Scholar 

Kang YB, Rawat S, Cirillo J, Bouchard M, Noh HM. Layered long-term co-culture of hepatocytes and endothelial cells on a transwell membrane: toward engineering the liver sinusoid. Biofabrication. 2013;5:045008.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Cunningham RP, Porat-Shliom N. Liver zonation—revisiting old questions with new technologies. Front Physiol. 2021;12:732929.

Article  PubMed  PubMed Central  Google Scholar 

Michalopoulos GK, Bhushan B. Liver regeneration: biological and pathological mechanisms and implications. Nat Rev Gastroenterol Hepatol. 2021;18:40–55.

Article  PubMed  Google Scholar 

Zhao L, Jin Y, Donahue K, Tsui M, Fish M, Logan CY, et al. Tissue repair in the mouse liver following acute carbon tetrachloride depends on injury-induced Wnt/beta-catenin signaling. Hepatology. 2019;69:2623–35.

Article  CAS  PubMed  Google Scholar 

Annunziato S, Sun T, Tchorz JS. The RSPO-LGR4/5-ZNRF3/RNF43 module in liver homeostasis, regeneration, and disease. Hepatology. 2022;76:888–99.

Article  CAS  Google Scholar 

Chang CF, Hsu LS, Weng CY, Chen CK, Wang SY, Chou YH, et al. N-glycosylation of human R-Spondin 1 is required for efficient secretion and stability but not for its heparin binding ability. Int J Mol Sci. 2016;17:937.

Article  PubMed  PubMed Central  Google Scholar 

Bao J, Fisher JE, Lillegard JB, Wang W, Amiot B, Yu Y, et al. Serum-free medium and mesenchymal stromal cells enhance functionality and stabilize integrity of rat hepatocyte spheroids. Cell Transplant. 2013;22:299–308.

Article  PubMed  Google Scholar 

Nyberg SL, Hardin J, Amiot B, Argikar UA, Remmel RP, Rinaldo P. Rapid, large-scale formation of porcine hepatocyte spheroids in a novel spheroid reservoir bioartificial liver. Liver Transpl. 2005;11:901–10.

Article  Google Scholar 

Sielaff TD, Hu MY, Rao S, Groehler K, Olson D, Mann HJ, et al. A technique for porcine hepatocyte harvest and description of differentiated metabolic functions in static culture. Transplantation. 1995;59:1459–63.

Article  CAS  Google Scholar 

Bell CC, Hendriks DF, Moro SM, Ellis E, Walsh J, Renblom A, et al. Characterization of primary human hepatocyte spheroids as a model system for drug-induced liver injury, liver function and disease. Sci Rep. 2016;6:25187.

Article  CAS  Google Scholar 

Wang G, Zheng Y, Wang Y, Cai Z, Liao N, Liu J, et al. Co-culture system of hepatocytes and endothelial cells: two in vitro approaches for enhancing liver-specific functions of hepatocytes. Cytotechnology. 2018;70:1279–90.

Article  CAS  Google Scholar 

Godoy P, Hewitt NJ, Albrecht U, Andersen ME, Ansari N, Bhattacharya S, et al. Recent advances in 2D and 3D in vitro systems using primary hepatocytes, alternative hepatocyte sources and non-parenchymal liver cells and their use in investigating mechanisms of hepatotoxicity, cell signaling and ADME. Arch Toxicol. 2013;87:1315–530.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Van As AB, Lotz Z, Tyler M, Adams S, Ryffel B, Kahn D. Histological assessment after different methods of reperfusion following liver transplantation. S Afr J Surg Suid-Afrikaanse tydskrif vir chirurgie. 2002;40:95–8.

Google Scholar 

Preziosi M, Okabe H, Poddar M, Singh S, Monga SP. Endothelial Wnts regulate beta-catenin signaling in murine liver zonation and regeneration: a sequel to the Wnt-Wnt situation. Hepatol Commun. 2018;2:845–60.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Salerno S, Campana C, Morelli S, Drioli E, De Bartolo L. Human hepatocytes and endothelial cells in organotypic membrane systems. Biomaterials. 2011;32:8848–59.

Article  CAS  PubMed  Google Scholar 

Asai A, Aihara E, Watson C, Mourya R, Mizuochi T, Shivakumar P, et al. Paracrine signals regulate human liver organoid maturation from induced pluripotent stem cells. Development. 2017;144:1056–64.

Article  CAS  Google Scholar 

Saviano A, Henderson NC, Baumert TF. Single-cell genomics and spatial transcriptomics: discovery of novel cell states and cellular interactions in liver physiology and disease biology. J Hepatol. 2020;73:1219–30.

Article 

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