Molecular mechanisms and biotechnology applications of CRISPR–Cas12a

Doudna, J. A. & Charpentier, E. The new frontier of genome engineering with CRISPR-Cas9. Science 346, 1258096 (2014).

Article  PubMed  Google Scholar 

Wright, A. V., Nuñez, J. K. & Doudna, J. A. Biology and applications of CRISPR systems: harnessing nature’s toolbox for genome engineering. Cell 164, 29–44 (2016).

Article  CAS  PubMed  Google Scholar 

Barrangou, R. & Horvath, P. A decade of discovery: CRISPR functions and applications. Nat. Microbiol. 2, 1–9 (2017).

Article  Google Scholar 

Knipping, F. et al. Genome-wide specificity of highly efficient TALENs and CRISPR/Cas9 for T cell receptor modification. Mol. Ther. Methods Clin. Dev. 4, 213–224 (2017).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Chandrasegaran, S. & Carroll, D. Origins of programmable nucleases for genome engineering. J. Mol. Biol. 428, 963–989 (2016).

Article  CAS  PubMed  Google Scholar 

Jinek, M. et al. A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Science 337, 816–821 (2012).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Gasiunas, G., Barrangou, R., Horvath, P. & Siksnys, V. Cas9-crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria. Proc. Natl Acad. Sci. USA 109, E2579–E2586 (2012).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Cong, L. et al. Multiplex genome engineering using CRISPR/Cas systems. Science 339, 819–823 (2013).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Wang, J. Y., Pausch, P. & Doudna, J. A. Structural biology of CRISPR–Cas immunity and genome editing enzymes. Nat. Rev. Microbiol. 20, 641–656 (2022).

Article  CAS  PubMed  Google Scholar 

Marraffini, L. A. CRISPR-Cas immunity in prokaryotes. Nature 526, 55–61 (2015).

Article  CAS  PubMed  Google Scholar 

Jinek, M. et al. Structures of Cas9 endonucleases reveal RNA-mediated conformational activation. Science 343, 1247997 (2014).

Article  PubMed  PubMed Central  Google Scholar 

Chen, F. et al. Recent advances of CRISPR-based genome editing for enhancing staple crops. Front. Plant Sci. 15, 1478398 (2024).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Lakhawat, S. S., Malik, N., Kumar, V., Kumar, S. & Sharma, P. K. Implications of CRISPR-Cas9 in developing next generation biofuel: a mini-review. Curr. Protein Pept. Sci. 23, 574–584 (2022).

Article  CAS  PubMed  Google Scholar 

Abdelnour, S. A., Xie, L., Hassanin, A. A., Zuo, E. & Lu, Y. The potential of CRISPR/Cas9 gene editing as a treatment strategy for inherited diseases. Front. Cell Dev. Biol. 9, 699597 (2021).

Article  PubMed  PubMed Central  Google Scholar 

Doudna, J. A. The promise and challenge of therapeutic genome editing. Nature 578, 229–236 (2020).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Zetsche, B. et al. Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system. Cell 163, 759–771 (2015).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Swarts, D. C., Oost, J. vander & Jinek, M. Structural basis for guide RNA processing and seed-dependent DNA targeting by CRISPR-Cas12a. Mol. Cell 66, 221–233.e4 (2017).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Swarts, D. C. & Jinek, M. Mechanistic insights into the cis- and trans-acting DNase activities of Cas12a. Mol. Cell 73, 589–600.e4 (2019).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Stella, S. et al. Conformational activation promotes CRISPR-Cas12a catalysis and resetting of the endonuclease activity. Cell 175, 1856–1871.e21 (2018).

Article  CAS  PubMed  Google Scholar 

Broughton, J. P. et al. CRISPR–Cas12-based detection of SARS-CoV-2. Nat. Biotechnol. 38, 870–874 (2020).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Deng, F. et al. Topological barrier to Cas12a activation by circular DNA nanostructures facilitates autocatalysis and transforms DNA/RNA sensing. Nat. Commun. 15, 1818 (2024).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Wani, A. K. et al. CRISPR/Cas12a-based biosensors for environmental monitoring and diagnostics. Environ. Technol. Innov. 34, 103625 (2024).

Article  CAS  Google Scholar 

Xu, X. et al. Molecular detection of Phytophthora cinnamomi by RPA-CRISPR/Cas12a-mediated isothermal amplification. Forests 15, 772 (2024).

Article  Google Scholar 

Nishimasu, H. & Nureki, O. Structures and mechanisms of CRISPR RNA-guided effector nucleases. Curr. Opin. Struct. Biol. 43, 68–78 (2017).

Article  CAS  PubMed  Google Scholar 

Yosef, I., Goren, M. G. & Qimron, U. Proteins and DNA elements essential for the CRISPR adaptation process in Escherichia coli. Nucleic Acids Res. 40, 5569–5576 (2012).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Levy, A. et al. CRISPR adaptation biases explain preference for acquisition of foreign DNA. Nature 520, 505–510 (2015).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Radovčić, M. et al. CRISPR–Cas adaptation in Escherichia coli requires RecBCD helicase but not nuclease activity, is independent of homologous recombination, and is antagonized by 5’ ssDNA exonucleases. Nucleic Acids Res. 46, 10173–10183 (2018).

PubMed  PubMed Central  Google Scholar 

Swarts, D. C., Mosterd, C., Van Passel, M. W. & Brouns, S. J. CRISPR interference directs strand specific spacer acquisition. PLoS ONE 7, e35888 (2012).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Datsenko, K. A. et al. Molecular memory of prior infections activates the CRISPR/Cas adaptive bacterial immunity system. Nat. Commun. 3, 945 (2012).

Article  PubMed  Google Scholar 

Staals, R. H. et al. Interference-driven spacer acquisition is dominant over naive and primed adaptation in a native CRISPR–Cas system. Nat. Commun. 7, 12853 (2016).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Mojica, F. J., Díez-Villaseñor, C., García-Martínez, J. & Soria, E. Intervening sequences of regularly spaced prokaryotic repeats derive from foreign genetic elements. J. Mol. Evol. 60, 174–182 (2005).

Article  CAS  PubMed  Google Scholar 

Comments (0)

No login
gif