Human Subcutaneous Derived Stromal Vascular Fraction Endothelial Cells Display Venous and Arterial Markers in Culture and 3D Capillaries

Krüger-Genge A, Blocki A, Franke R-P, Jung F. Vascular endothelial cell biology: an update. Int J Mol Sci. 2019;20:4411.

Article  PubMed  PubMed Central  Google Scholar 

Wolf K, Hu H, Isaji T, Dardik A. Molecular identity of arteries, veins, and lymphatics. J Vasc Surg. 2019;69:253–62.

Article  PubMed  Google Scholar 

Hirashima M, Suda T. Differentiation of arterial and venous endothelial cells and vascular morphogenesis. Endothelium. 2006;13:137–45.

Article  CAS  PubMed  Google Scholar 

Klar AS, Biedermann T, Simmen-Meuli C, Reichmann E, Meuli M. Comparison of in vivo immune responses following transplantation of vascularized and non-vascularized human dermo-epidermal skin substitutes. Pediatr Surg Int. 2017;33:377–82.

Article  PubMed  Google Scholar 

Oualla-Bachiri W, Fernández-González A, Quiñones-Vico MI, Arias-Santiago S. From grafts to human bioengineered vascularized skin substitutes. Int J Mol Sci. 2020;21:8197.

Article  PubMed  PubMed Central  Google Scholar 

Pontiggia L, Van Hengel IAJ, Klar A, Rütsche D, Nanni M, Scheidegger A, et al. Bioprinting and plastic compression of large pigmented and vascularized human dermo-epidermal skin substitutes by means of a new robotic platform. J Tissue Eng. 2022:13:20417314221088513.

Miyazaki H, Tsunoi Y, Akagi T, Sato S, Akashi M, Saitoh D. A novel strategy to engineer pre-vascularized 3-dimensional skin substitutes to achieve efficient, functional engraftment. Sci Rep. 2019;9:7797.

Article  PubMed  PubMed Central  Google Scholar 

Monsuur HN, Weijers EM, Niessen FB, Gefen A, Koolwijk P, Gibbs S, et al. Extensive characterization and comparison of endothelial cells derived from dermis and adipose tissue: potential use in tissue engineering. PLoS ONE. 2016;11:e0167056.

Article  PubMed  PubMed Central  Google Scholar 

Simonacci F, Bertozzi N, Grieco MP, Raposio E. From liposuction to adipose-derived stem cells: indications and technique. Acta Biomed. 2019;90:197–208.

CAS  PubMed  PubMed Central  Google Scholar 

Klar AS, Güven S, Zimoch J, Zapiórkowska NA, Biedermann T, Böttcher-Haberzeth S, et al. Characterization of vasculogenic potential of human adipose-derived endothelial cells in a three-dimensional vascularized skin substitute. Pediatr Surg Int. 2016;32:17–27.

Article  PubMed  Google Scholar 

Klar AS, Güven S, Biedermann T, Luginbühl J, Böttcher-Haberzeth S, Meuli-Simmen C, et al. Tissue-engineered dermo-epidermal skin grafts prevascularized with adipose-derived cells. Biomaterials. 2014;35:5065–78.

Article  CAS  PubMed  Google Scholar 

Nilforoushzadeh MA, Sisakht MM, Amirkhani MA, Seifalian AM, Banafshe HR, Verdi J, et al. Engineered skin graft with stromal vascular fraction cells encapsulated in fibrin–collagen hydrogel: a clinical study for diabetic wound healing. J Tissue Eng Regen Med. 2020;14:424–40.

Article  CAS  PubMed  Google Scholar 

Muller WA, Ratti CM, McDonnell SL, Cohn ZA. A human endothelial cell-restricted, externally disposed plasmalemmal protein enriched in intercellular junctions. J Exp Med. 1989;170:399–414.

Article  CAS  PubMed  PubMed Central  Google Scholar 

He Y, Tacconi C, Dieterich LC, Kim J, Restivo G, Gousopoulos E, et al. Novel blood vascular endothelial subtype-specific markers in human skin unearthed by single-cell transcriptomic profiling. Cells. 2022;11:1111.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Guo L, Zhang H, Hou Y, Wei T, Liu J. Plasmalemma vesicle-associated protein: a crucial component of vascular homeostasis. Exp Ther Med. 2016;12:1639–44.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Herzog Y, Kalcheim C, Kahane N, Reshef R, Neufeld G. Differential expression of neuropilin-1 and neuropilin-2 in arteries and veins. Mech Dev. 2001;109:115–9.

Article  CAS  PubMed  Google Scholar 

Hong Y, Harvey N, Noh Y, Schacht V, Hirakawa S, Detmar M, et al. Prox1 is a master control gene in the program specifying lymphatic endothelial cell fate. Dev Dyn. 2002;225:351–7.

Article  CAS  PubMed  Google Scholar 

Jeong DP, Hall E, Neu E, Hanjaya-Putra D. Podoplanin is responsible for the distinct blood and lymphatic capillaries. Cell Mol Bioeng. 2022;15:467–78.

Article  CAS  PubMed  PubMed Central  Google Scholar 

You L-R, Lin F-J, Lee CT, DeMayo FJ, Tsai M-J, Tsai SY. Suppression of notch signalling by the COUP-TFII transcription factor regulates vein identity. Nature. 2005;435:98–104.

Article  CAS  PubMed  Google Scholar 

Nanni M, Rütsche D, Bächler C, Pontiggia L, Klar AS, Moehrlen U, et al. CD146 expression profile in human skin and pre-vascularized dermo-epidermal skin substitutes in vivo. J Biol Eng. 2023;17:9.

Marin V, Kaplanski G, Grès S, Farnarier C, Bongrand P. Endothelial cell culture: protocol to obtain and cultivate human umbilical endothelial cells. J Immunol Methods. 2001;254:183–90.

Article  CAS  PubMed  Google Scholar 

Richard L, Velasco P, Detmar M. A simple immunomagnetic protocol for the selective isolation and long-term culture of human dermal microvascular endothelial cells. Exp Cell Res. 1998;240:1–6.

Article  CAS  PubMed  Google Scholar 

Bateman ME, Strong AL, Gimble JM, Bunnell BA. Concise review: using fat to fight disease: a systematic review of nonhomologous adipose-derived stromal/stem cell therapies. Stem Cells. 2018;36:1311–28.

Article  PubMed  Google Scholar 

Ramakrishnan VM, Boyd NL. The adipose stromal vascular fraction as a complex cellular source for tissue engineering applications. Tissue Eng Part B Rev. 2018;24:289–99.

Article  PubMed  Google Scholar 

de Redondo PAG, Gubert F, Zaverucha-do-Valle C, Dutra TPP, de Ayres-Silva JP, Fernandes N, et al. Lymphatic vessels in human adipose tissue. Cell Tissue Res. 2020;379:511–20.

Article  PubMed  Google Scholar 

Vijay J, Gauthier M-F, Biswell RL, Louiselle DA, Johnston JJ, Cheung WA, et al. Single-cell analysis of human adipose tissue identifies depot- and disease-specific cell types. Nat Metab. 2019;2:97–109.

Article  PubMed  PubMed Central  Google Scholar 

Lassance L, Miedl H, Konya V, Heinemann A, Ebner B, Hackl H, et al. Differential response of arterial and venous endothelial cells to extracellular matrix is modulated by oxygen. Histochem Cell Biol. 2012;137:641–55.

Article  CAS  PubMed  Google Scholar 

Afshar Y, Ma F, Quach A, Jeong A, Sunshine HL, Freitas V, et al. Transcriptional drifts associated with environmental changes in endothelial cells. Elife. 2023;12:81370.

Article  Google Scholar 

Mack JJ, Mosqueiro TS, Archer BJ, Jones WM, Sunshine H, Faas GC, et al. NOTCH1 is a mechanosensor in adult arteries. Nat Commun. 2017;8:1620.

Article  PubMed  PubMed Central  Google Scholar 

Abbas ZN, Al-Saffar AZ, Jasim SM, Sulaiman GM. Comparative analysis between 2D and 3D colorectal cancer culture models for insights into cellular morphological and transcriptomic variations. Sci Rep. 2023;13:18380.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Rapp J, Ness J, Wolf J, Hospach A, Liang P, Hug MJ, et al. 2D and 3d in vitro angiogenesis assays highlight different aspects of angiogenesis. Biochimica et Biophysica Acta (BBA). 2024;1870:167028.

Article  CAS  Google Scholar 

Renò F, Sabbatini M. Breaking a vicious circle: lymphangiogenesis as a new therapeutic target in wound healing. Biomedicines. 2023;11:656.

Article  PubMed  PubMed Central  Google Scholar 

Rütsche D, Nanni M, Cheng P, Caflisch N, Tastanova A, Jenni C, et al. Human dermal microvascular arterial and venous blood endothelial cells and their use in bioengineered dermo‐epidermal skin substitutes. Small Methods. 2025;9:e2401588.

Article  PubMed 

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