Therapeutic Effects of N-Acetylcysteine-Primed, Iron Oxide Nanoparticle-Enhanced Mesenchymal Stem Cell Exosomes in Ototoxicity Hearing Loss

Deafness and Hearing loss. World Health Organization. 2024.

Batsaikhan T, Choi JS, Ha SM, Ahn Y, Seo YJ. D-galactose and hypoxia induce the early onset of age-related hearing loss deterioration in a mouse model. Tissue Eng Regen Med. 2023;20:779–87.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Reynard P, Thai-Van H. Drug-induced hearing loss: listening to the latest advances. Therapie. 2024;79:283–95.

Article  PubMed  Google Scholar 

Ryu KH, Cho KA, Park HS, Kim JY, Woo SY, Jo I, et al. Tonsil-derived mesenchymal stromal cells: evaluation of biologic, immunologic and genetic factors for successful banking. Cytotherapy. 2012;14:1193–202.

Article  CAS  PubMed  Google Scholar 

Bacic A, Prgomet D, Janjanin S. Tonsil-derived mesenchymal stem cells exert immunosuppressive effects on T cells. Croat Med J. 2019;60:12–9.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Cho KA, Lee HJ, Jeong H, Kim M, Jung SY, Park HS, et al. Tonsil-derived stem cells as a new source of adult stem cells. World J Stem Cells. 2019;11:506–18.

Article  PubMed  PubMed Central  Google Scholar 

Oh SY, Choi YM, Kim HY, Park YS, Jung SC, Park JW, et al. Application of tonsil-derived mesenchymal stem cells in tissue regeneration: concise review. Stem Cells. 2019;37:1252–60.

Article  PubMed  PubMed Central  Google Scholar 

Ahn YJ, Yun WS, Choi JS, Kim WC, Lee SH, Park DJ, et al. Biodistribution of poly clustered superparamagnetic iron oxide nanoparticle labeled mesenchymal stem cells in aminoglycoside induced ototoxic mouse model. Biomed Eng Lett. 2021;11:39–53.

Article  PubMed  PubMed Central  Google Scholar 

Chakraborty A, Jana NR. Vitamin c-conjugated nanoparticle protects cells from oxidative stress at low doses but induces oxidative stress and cell death at high doses. ACS Appl Mater Interfaces. 2017;9:41807–17.

Article  CAS  PubMed  Google Scholar 

Chung TW, Wu TY, Siah ZY, Liu DZ. Antioxidative nac-loaded silk nanoparticles with opening mucosal tight junctions for nasal drug delivery: an in vitro and in vivo study. Pharmaceutics. 2022;14:1288.

Article  PubMed  PubMed Central  Google Scholar 

Bai X, Wang M, Niu X, Yu H, Yue JX, Sun Y. Effect of n-acetyl-cysteine treatment on sensorineural hearing loss: a meta-analysis. World J Otorhinolaryngol Head Neck Surg. 2022;8:205–12.

Article  PubMed  PubMed Central  Google Scholar 

Bai X, Chen S, Xu K, Jin Y, Niu X, Xie L, et al. N-acetylcysteine combined with dexamethasone treatment improves sudden sensorineural hearing loss and attenuates hair cell death caused by ros stress. Front Cell Dev Biol. 2021;9:659486.

Article  PubMed  PubMed Central  Google Scholar 

Orgel E, Knight KR, Chi YY, Malvar J, Rushing T, Mena V, et al. Intravenous n-acetylcysteine to prevent cisplatin-induced hearing loss in children: a nonrandomized controlled phase I trial. Clin Cancer Res. 2023;29:2410–8.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Zadrozniak M, Szymanski M, Luszczki JJ. N-acetyl-l-cysteine affects ototoxicity evoked by amikacin and furosemide either alone or in combination in a mouse model of hearing threshold decrease. Int J Mol Sci. 2023;24.

Li D, Ding Z, Du K, Ye X, Cheng S. Reactive oxygen species as a link between antioxidant pathways and autophagy. Oxid Med Cell Longev. 2021;2021:5583215.

Article  PubMed  PubMed Central  Google Scholar 

Zhang L, Zhu C, Huang R, Ding Y, Ruan C, Shen XC. Mechanisms of reactive oxygen species generated by inorganic nanomaterials for cancer therapeutics. Front Chem. 2021;9:630969.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Giustarini D, Milzani A, Dalle-Donne I, Tsikas D, Rossi R. N-acetylcysteine ethyl ester (NACET): a novel lipophilic cell-permeable cysteine derivative with an unusual pharmacokinetic feature and remarkable antioxidant potential. Biochem Pharmacol. 2012;84:1522–33.

Article  CAS  PubMed  Google Scholar 

Pedre B, Barayeu U, Ezerina D, Dick TP. The mechanism of action of n-acetylcysteine (NAC): the emerging role of H(2)S and sulfane sulfur species. Pharmacol Ther. 2021;228:107916.

Article  CAS  PubMed  Google Scholar 

Zhang K, Cheng K. Stem cell-derived exosome versus stem cell therapy. Nat Rev Bioeng. 2023. https://doi.org/10.1038/s44222-023-00064-2.

Article  PubMed  PubMed Central  Google Scholar 

Organization WH. WHO consolidated guidelines on tuberculosis. Module 4: treatment-drug resistance tuberculosis treatment. 2020.

Tyuryaeva I, Lyublinskaya O. Expected and unexpected effects of pharmacological antioxidants. Int J Mol Sci. 2023;24:9303.

Article  PubMed  PubMed Central  Google Scholar 

Galaris D, Barbouti A, Pantopoulos K. Iron homeostasis and oxidative stress: an intimate relationship. Biochim Biophys Acta Mol Cell Res. 2019;1866:118535.

Article  CAS  Google Scholar 

Yu Y, Yan Y, Niu F, Wang Y, Chen X, Su G, et al. Ferroptosis: a cell death connecting oxidative stress, inflammation and cardiovascular diseases. Cell Death Discov. 2021;7:193.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Misra S, Boylan M, Selvam A, Spallholz JE, Bjornstedt M. Redox-active selenium compounds--from toxicity and cell death to cancer treatment. Nutrients. 2015;7:3536–56.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Kim J, Gong YX, Jeong EM. Measuring glutathione regeneration capacity in stem cells. Int J Stem Cells. 2023;16:356–62.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Lim J, Heo J, Ju H, Shin JW, Kim Y, Lee S, et al. Glutathione dynamics determine the therapeutic efficacy of mesenchymal stem cells for graft-versus-host disease via CREB1-NRF2 pathway. Sci Adv. 2020;6:1334.

Article  Google Scholar 

Hopkins BL, Nadler M, Skoko JJ, Bertomeu T, Pelosi A, Shafaei PM, et al. A peroxidase peroxiredoxin 1-specific redox regulation of the novel FOXO3 microRNA target let-7. Antioxid Redox Signal. 2018;28:62–77.

Article  CAS  PubMed  Google Scholar 

Moriwaki T, Yoshimura A, Tamari Y, Sasanuma H, Takeda S, Seki M, et al. PRDX1 is essential for the viability and maintenance of reactive oxygen species in chicken DT40. Genes Environ. 2021;43:35.

Article  PubMed  PubMed Central  Google Scholar 

Bodega G, Alique M, Puebla L, Carracedo J, Ramirez RM. Microvesicles: ros scavengers and ros producers. J Extracell Vesicles. 2019;8:1626654.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Li H, Steyger PS. Systemic aminoglycosides are trafficked via endolymph into cochlear hair cells. Sci Rep. 2011;1:159.

Article  PubMed  PubMed Central  Google Scholar 

Wang Q, Zhu H, Zhou WG, Guo XC, Wu MJ, Xu ZY, et al. N-acetylcysteine-pretreated human embryonic mesenchymal stem cell administration protects against bleomycin-induced lung injury. Am J Med Sci. 2013;346:113–22.

Article  PubMed  Google Scholar 

Barzegari A, Omidi Y, Landon R, Gueguen V, Parvizpour S, Meddahi-Pelle A, et al. The protective effect of n-acetylcysteine on antimycin A-induced respiratory chain deficiency in mesenchymal stem cells. Chem Biol Interact. 2022;360:109937.

Article  CAS  PubMed  Google Scholar 

Yang W, Wang L, Mettenbrink EM, DeAngelis PL, Wilhelm S. Nanoparticle toxicology. Annu Rev Pharmacol Toxicol. 2021;61:269–89.

Article  CAS  P

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