Denk, W., Strickler, J. H. & Webb, W. W. Two-photon laser scanning fluorescence microscopy. Science 248, 73–76 (1990).

Article  CAS  PubMed  Google Scholar 

Kleinfeld, D., Mitra, P. P., Helmchen, F. & Denk, W. Fluctuations and stimulus-induced changes in blood flow observed in individual capillaries in layers 2 through 4 of rat neocortex. Proc. Natl Acad. Sci. USA 95, 15741–15746 (1998).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Svoboda, K., Denk, W., Kleinfeld, D. & Tank, D. W. In vivo dendritic calcium dynamics in neocortical pyramidal neurons. Nature 385, 161–165 (1997).

Article  CAS  PubMed  Google Scholar 

Dong, C. et al. Fluorescence imaging of neural activity, neurochemical dynamics, and drug-specific receptor conformation with genetically encoded sensors. Annu. Rev. Neurosci. 45, 273–294 (2022).

Article  PubMed  PubMed Central  Google Scholar 

Chen, T. W. et al. Ultrasensitive fluorescent proteins for imaging neuronal activity. Nature 499, 295–300 (2013).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Marvin, J. S. et al. Stability, affinity, and chromatic variants of the glutamate sensor iGluSnFR. Nat. Methods 15, 936–939 (2018).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Aggarwal, A. et al. Glutamate indicators with improved activation kinetics and localization for imaging synaptic transmission. Nat. Methods 20, 925–935 (2023).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Villette, V. et al. Ultrafast two-photon imaging of a high-gain voltage indicator in awake behaving mice. Cell 179, 1590–1608 e23 (2019).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Kleinfeld, D. et al. Can one concurrently record electrical spikes from every neuron in a mammalian brain? Neuron 103, 1005–1015 (2019).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Jun, J. J. et al. Fully integrated silicon probes for high-density recording of neural activity. Nature 551, 232–236 (2017).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Ohki, K., Chung, S., Ch’ng, Y. H., Kara, P. & Reid, R. C. Functional imaging with cellular resolution reveals precise micro-architecture in visual cortex. Nature 433, 597–603 (2005).

Article  CAS  PubMed  Google Scholar 

Xu, S. et al. Behavioral state coding by molecularly defined paraventricular hypothalamic cell type ensembles. Science 370, eabb2494 (2020).

Article  CAS  PubMed  Google Scholar 

Hampson, K. M. et al. Adaptive optics for high-resolution imaging. Nat. Rev. Methods Prim. 1, 68 (2021).

Article  CAS  Google Scholar 

Wang, K. et al. Direct wavefront sensing for high-resolution in vivo imaging in scattering tissue. Nat. Commun. 6, 7276 (2015).

Article  CAS  PubMed  Google Scholar 

Wang, K. et al. Rapid adaptive optical recovery of optimal resolution over large volumes. Nat. Methods 11, 625–628 (2014).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Liu, R., Li, Z., Marvin, J. S. & Kleinfeld, D. Direct wavefront sensing enables functional imaging of infragranular axons and spines. Nat. Methods 16, 615–618 (2019).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Debarre, D. et al. Image-based adaptive optics for two-photon microscopy. Opt. Lett. 34, 2495–2497 (2009).

Article  PubMed  PubMed Central  Google Scholar 

Tang, J., Germain, R. N. & Cui, M. Superpenetration optical microscopy by iterative multiphoton adaptive compensation technique. Proc. Natl Acad. Sci. USA 109, 8434–8439 (2012).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Ji, N., Milkie, D. E. & Betzig, E. Adaptive optics via pupil segmentation for high-resolution imaging in biological tissues. Nat. Methods 7, 141–147 (2010).

Article  CAS  PubMed  Google Scholar 

Streich, L. et al. High-resolution structural and functional deep brain imaging using adaptive optics three-photon microscopy. Nat. Methods 18, 1253–1258 (2021).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Rodriguez, C. et al. An adaptive optics module for deep tissue multiphoton imaging in vivo. Nat. Methods 18, 1259–1264 (2021).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Aviles-Espinosa, R. et al. Measurement and correction of in vivo sample aberrations employing a nonlinear guide-star in two-photon excited fluorescence microscopy. Biomed. Opt. Express 2, 3135–3149 (2011).

Article  PubMed  PubMed Central  Google Scholar 

Yardeni, T., Eckhaus, M., Morris, H. D., Huizing, M. & Hoogstraten-Miller, S. Retro-orbital injections in mice. Lab Anim. (NY) 40, 155–160 (2011).

Article  PubMed  Google Scholar 

Drew, P. J. et al. Chronic optical access through a polished and reinforced thinned skull. Nat. Methods 7, 981–984 (2010).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Holtmaat, A. et al. Long-term, high-resolution imaging in the mouse neocortex through a chronic cranial window. Nat. Protoc. 4, 1128–1144 (2009).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Ji, N., Sato, T. R. & Betzig, E. Characterization and adaptive optical correction of aberrations during in vivo imaging in the mouse cortex. Proc. Natl Acad. Sci. USA 109, 22–27 (2012).

Article  CAS  PubMed  Google Scholar 

Ji, N. Adaptive optical fluorescence microscopy. Nat. Methods 14, 374–380 (2017).

Article  CAS  PubMed  Google Scholar 

Wang, C. et al. Multiplexed aberration measurement for deep tissue imaging in vivo. Nat. Methods 11, 1037–1040 (2014).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Qin, Z. et al. Adaptive optics two-photon microscopy enables near-diffraction-limited and functional retinal imaging in vivo. Light Sci. Appl. 9, 79 (2020).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Meng, G. et al. High-throughput synapse-resolving two-photon fluorescence microendoscopy for deep-brain volumetric imaging in vivo. eLife 8, e40805 (2019).

Article  PubMed  PubMed Central  Google Scholar 

Resendez, S. L. et al. Visualization of cortical, subcortical and deep brain neural circuit dynamics during naturalistic mammalian behavior with head-mounted microscopes and chronically implanted lenses. Nat. Protoc. 11, 566–597 (2016).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Qin, Z. et al. Adaptive optics two-photon endomicroscopy enables deep-brain imaging at synaptic resolution over large volumes. Sci. Adv. 6, eabc6521 (2020).

Article  PubMed  PubMed Central  Google Scholar 

Noll, R. J. Zernike polynomials and atmospheric-turbulence. J. Opt. Soc. Am. 66, 207–211 (1976).

Article  Google Scholar 

Berlage, C. et al. Deep tissue scattering compensation with three-photon F-SHARP. Optica 8, 1613–1619 (2021).

Article  Google Scholar 

Papadopoulos, I. N., Jouhanneau, J.-S., Poulet, J. F. A. & Judkewitz, B. Scattering compensation by focus scanning holographic aberration probing (F-SHARP). Nat. Photonics 11, 116–123 (2016).

Article  Google Scholar 

Podgorski, K. & Ranganathan, G. Brain heating induced by near-infrared lasers during multiphoton microscopy. J. Neurophysiol. 116, 1012–1023 (2016).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Prevedel, R. et al. Fast volumetric calcium imaging across multiple cortical layers using sculpted light. Nat. Methods 13, 1021–1028 (2016).

Article  CAS  PubMed  PubMed Central