Photothermal nanofiber-mediated photoporation for gentle and efficient intracellular delivery of macromolecules

Stewart, M. P. et al. In vitro and ex vivo strategies for intracellular delivery. Nature 538, 183–192 (2016).

Article  CAS  PubMed  Google Scholar 

Morshedi Rad, D. et al. A comprehensive review on intracellular delivery. Adv. Mater. 33, 2005363 (2021).

Article  CAS  Google Scholar 

Zhou, P. et al. In vivo discovery of immunotherapy targets in the tumour microenvironment. Nature 506, 52–57 (2014).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Wang, X. & Rivière, I. Clinical manufacturing of CAR T cells: foundation of a promising therapy. Mol. Ther. Oncolytics 3, 16015 (2016).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Zhang, C., Hu, Y., Xiao, W. & Tian, Z. Chimeric antigen receptor- and natural killer cell receptor-engineered innate killer cells in cancer immunotherapy. Cell. Mol. Immunol. 18, 2083–2100 (2021).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Sadelain, M., Rivière, I. & Riddell, S. Therapeutic T cell engineering. Nature 545, 423–431 (2017).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Cox, D. B. T., Platt, R. J. & Zhang, F. Therapeutic genome editing: prospects and challenges. Nat. Med. 21, 121–131 (2015).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Stewart, M. P., Langer, R. & Jensen, K. F. Intracellular delivery by membrane disruption: mechanisms, strategies, and concepts. Chem. Rev. 118, 7409–7531 (2018).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Houthaeve, G., De Smedt, S. C., Braeckmans, K. & De Vos, W. H. The cellular response to plasma membrane disruption for nanomaterial delivery. Nano Converg. 9, 6 (2022).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Barber, M. A. A technic for the inoculation of bacteria and other substances into living cells. J. Infect. Dis. 8, 348–360 (1911).

Article  Google Scholar 

June, C. H. & Sadelain, M. Chimeric antigen receptor therapy. New Engl. J. Med. 379, 64–73 (2018).

Article  CAS  PubMed  Google Scholar 

Butt, M. H. et al. Appraisal for the potential of viral and nonviral vectors in gene therapy: a review. Genes 13, 1370 (2022).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Yin, H. et al. Non-viral vectors for gene-based therapy. Nat. Rev. Genet. 15, 541–555 (2014).

Article  CAS  PubMed  Google Scholar 

Du, X. et al. Advanced physical techniques for gene delivery based on membrane perforation. Drug Deliv. 25, 1516–1525 (2018).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Aghaamoo, M. et al. High-throughput and dosage-controlled intracellular delivery of large cargos by an acoustic-electric micro-vortices platform. Adv. Sci. 9, 2102021 (2022).

Article  CAS  Google Scholar 

Xiong, R. et al. Laser-assisted photoporation: fundamentals, technological advances and applications. Adv. Phys. X 1, 596–620 (2016).

CAS  Google Scholar 

Mitchell, M. J. et al. Engineering precision nanoparticles for drug delivery. Nat. Rev. Drug Discov. 20, 101–124 (2021).

Article  CAS  PubMed  Google Scholar 

Tay, A. & Melosh, N. Transfection with nanostructure electro-injection is minimally perturbative. Adv. Therap. 2, 1900133 (2019).

Article  CAS  Google Scholar 

Cao, Y. et al. Nontoxic nanopore electroporation for effective intracellular delivery of biological macromolecules. Proc. Natl Acad. Sci. USA 116, 7899–7904 (2019).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Xiong, R. et al. Photothermal nanomaterial-mediated photoporation. Acc. Chem. Res. 56, 631–643 (2023).

Article  CAS  PubMed  Google Scholar 

Xiong, R. et al. Comparison of gold nanoparticle mediated photoporation: vapor nanobubbles outperform direct heating for delivering macromolecules in live cells. ACS Nano 8, 6288–6296 (2014).

Article  CAS  PubMed  Google Scholar 

Ramon, J., Xiong, R., De Smedt, S. C., Raemdonck, K. & Braeckmans, K. Vapor nanobubble-mediated photoporation constitutes a versatile intracellular delivery technology. Curr. Opin. Colloid Interface Sci. 54, 101453 (2021).

Article  CAS  Google Scholar 

Lapotko, D. Plasmonic nanoparticle-generated photothermal bubbles and their biomedical applications. Nanomedicine 4, 813–845 (2009).

Article  CAS  PubMed  Google Scholar 

Raes, L. et al. Gold nanoparticle-mediated photoporation enables delivery of macromolecules over a wide range of molecular weights in human CD4+ T cells. Crystals 9, 411 (2019).

Article  CAS  Google Scholar 

Raes, L. et al. Cas9 RNP transfection by vapor nanobubble photoporation for ex vivo cell engineering. Mol. Ther. Nucleic. Acids 25, 696–707 (2021).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Siegrist, S. et al. Preclinical hazard evaluation strategy for nanomedicines. Nanotoxicology 13, 73–99 (2019).

Article  CAS  PubMed  Google Scholar 

Foulkes, R. et al. The regulation of nanomaterials and nanomedicines for clinical application: current and future perspectives. Biomater. Sci. 8, 4653–4664 (2020).

Article  CAS  PubMed  Google Scholar 

Soenen, S. J. et al. Cytotoxic effects of gold nanoparticles: a multiparametric study. ACS Nano 6, 5767–5783 (2012).

Article  CAS  PubMed  Google Scholar 

Xiong, R. et al. Photothermal nanofibres enable safe engineering of therapeutic cells. Nat. Nanotechnol. 16, 1281–1291 (2021).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Berdecka, D. et al. Photothermal nanofibers enable macromolecule delivery in unstimulated human T cells. Appl. Mater. Today 35, 101991 (2023).

Article  Google Scholar 

Hua, D. et al. Bubble forming films for spatial selective cell killing. Adv. Mater. 33, 2008379 (2021).

Article  CAS  Google Scholar 

Zhang, Z., Qiu, S., Zhang, X. & Chen, W. Optimized DNA electroporation for primary human T cell engineering. BMC Biotechnol. 18, 4 (2018).

Article  PubMed  PubMed Central  Google Scholar 

Costa, M. et al. A method for genetic modification of human embryonic stem cells using electroporation. Nat. Protoc. 2, 792–796 (2007).

Article  CAS  PubMed 

Comments (0)

No login
gif