Labeling, isolation and characterization of cell-type-specific exosomes derived from mouse skin tissue

Zappulli, V., Friis, K. P., Fitzpatrick, Z., Maguire, C. A. & Breakefield, X. O. Extracellular vesicles and intercellular communication within the nervous system. J. Clin. Invest. 126, 1198–high–1207 (2016).

Article  Google Scholar 

Colombo, M., Raposo, G. & Thery, C. Biogenesis, secretion, and intercellular interactions of exosomes and other extracellular vesicles. Annu. Rev. Cell Dev. Biol. 30, 255–289 (2014).

Article  CAS  PubMed  Google Scholar 

Faict, S. et al. Exosomes play a role in multiple myeloma bone disease and tumor development by targeting osteoclasts and osteoblasts. Blood Cancer J. 8, 105 (2018).

Article  PubMed  PubMed Central  Google Scholar 

Hosseinkhani, B., Kuypers, S., van den Akker, N. M. S., Molin, D. G. M. & Michiels, L. Extracellular vesicles work as a functional inflammatory mediator between vascular endothelial cells and immune cells. Front. Immunol. 9, 1789 (2018).

Article  PubMed  PubMed Central  Google Scholar 

Wang, J. et al. Extracellular vesicle cross-talk in the bone marrow microenvironment: implications in multiple myeloma. Oncotarget 7, 38927–38945 (2016).

Article  PubMed  PubMed Central  Google Scholar 

Xu, R., Greening, D. W., Zhu, H. J., Takahashi, N. & Simpson, R. J. Extracellular vesicle isolation and characterization: toward clinical application. J. Clin. Invest. 126, 1152–1162 (2016).

Article  PubMed  PubMed Central  Google Scholar 

Tkach, M. & Théry, C. Communication by extracellular vesicles: where we are and where we need to go. Cell 164, 1226–1232 (2016).

Article  CAS  PubMed  Google Scholar 

Visnovitz, T. Extracellular vesicles: biology and therapeutic applications. Int. J. Mol. Sci 25, 13034 (2024).

Article  PubMed  PubMed Central  Google Scholar 

Srivastava, A. et al. Progress in extracellular vesicle biology and their application in cancer medicine. Wiley Interdiscip. Rev. Nanomed. Nanobiotechnol. 12, e1621 (2020).

Article  PubMed  PubMed Central  Google Scholar 

Lin, J., Yang, Z., Wang, L., Xing, D. & Lin, J. Global research trends in extracellular vesicles based on stem cells from 1991 to 2021: a bibliometric and visualized study. Front. Bioeng. Biotechnol. 10, 956058 (2022).

Article  PubMed  PubMed Central  Google Scholar 

Kelwick, R. J. R., Webb, A. J., Heliot, A., Segura, C. T. & Freemont, P. S. Opportunities to accelerate extracellular vesicle research with cell-free synthetic biology. J. Extracell. Biol. 2, e90 (2023).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Welsh, J. A. et al. Minimal information for studies of extracellular vesicles (MISEV2023): from basic to advanced approaches. J. Extracell. Vesicles 13, e12404 (2024).

Article  PubMed  PubMed Central  Google Scholar 

Watanabe, S. et al. Skeletal muscle releases extracellular vesicles with distinct protein and microRNA signatures that function in the muscle microenvironment. PNAS Nexus 1, pgac173 (2022).

Article  PubMed  PubMed Central  Google Scholar 

Thakur, A. et al. The mini player with diverse functions: extracellular vesicles in cell biology, disease, and therapeutics. Protein Cell 13, 631–654 (2021).

Article  PubMed  PubMed Central  Google Scholar 

Nelson, B. C. et al. Measurement and standardization challenges for extracellular vesicle therapeutic delivery vectors. Nanomedicine 15, 2149–2170 (2020).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Yadav, A., Xuan, Y., Sen, C. K. & Ghatak, S. Standardized reporting of research on exosomes to ensure rigor and reproducibility. Adv. Wound Care 13, 584–599 (2024).

Article  CAS  Google Scholar 

Rankin-Turner, S. et al. A call for the standardised reporting of factors affecting the exogenous loading of extracellular vesicles with therapeutic cargos. Adv. Drug Deliv. Rev. 173, 479–491 (2021).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Thery, C., Ostrowski, M. & Segura, E. Membrane vesicles as conveyors of immune responses. Nat. Rev. Immunol. 9, 581–593 (2009).

Article  CAS  PubMed  Google Scholar 

Doyle, L. M. & Wang, M. Z. Overview of extracellular vesicles, their origin, composition, purpose, and methods for exosome isolation and analysis. Cells 8, 727 (2019).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Kalluri, R. & LeBleu, V. S. The biology, function, and biomedical applications of exosomes. Science 367, eaau6977 (2020).

Article  CAS  PubMed  PubMed Central  Google Scholar 

van Niel, G., D'Angelo, G. & Raposo, G. Shedding light on the cell biology of extracellular vesicles. Nat. Rev. Mol. Cell Biol 19, 213–228 (2018).

Article  CAS  PubMed  Google Scholar 

Jeppesen, D. K., Zhang, Q., Franklin, J. L. & Coffey, R. J. Extracellular vesicles and nanoparticles: emerging complexities. Trends Cell Biol 33, 667–681 (2023).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Pérez-Boza, J., Lion, M. & Struman, I. Exploring the RNA landscape of endothelial exosomes. RNA 24, 423–435 (2018).

Article  PubMed  PubMed Central  Google Scholar 

Villarroya-Beltri, C. et al. Sumoylated hnRNPA2B1 controls the sorting of miRNAs into exosomes through binding to specific motifs. Nat. Commun. 4, 2980 (2013).

Article  PubMed  Google Scholar 

Janas, T., Janas, M. M., Sapoń, K. & Janas, T. Mechanisms of RNA loading into exosomes. FEBS Lett. 589, 1391–1398 (2015).

Article  CAS  PubMed  Google Scholar 

Creemers, E. E., Tijsen, A. J. & Pinto, Y. M. Circulating microRNAs: novel biomarkers and extracellular communicators in cardiovascular disease. Circ. Res. 110, 483–495 (2012).

Article  CAS  PubMed  Google Scholar 

Guduric-Fuchs, J. et al. Selective extracellular vesicle-mediated export of an overlapping set of microRNAs from multiple cell types. BMC Genom. 13, 357 (2012).

Article  CAS  Google Scholar 

Zhang, J. et al. Exosome and exosomal microRNA: trafficking, sorting, and function. Genom. Proteom. Bioinform. 13, 17–24 (2015).

Article  CAS  Google Scholar 

Van Deun, J. et al. EV-TRACK: transparent reporting and centralizing knowledge in extracellular vesicle research. Nat. Methods 14, 228–232 (2017).

Article  PubMed  Google Scholar 

Van Deun, J. et al. The impact of disparate isolation methods for extracellular vesicles on downstream RNA profiling. J. Extracell. Vesicles 3, 24858 (2014).

Article  Google Scholar 

Kowal, J. et al. Proteomic comparison defines novel markers to characterize heterogeneous populations of extracellular vesicle subtypes. Proc. Natl Acad. Sci. USA 113, E968–E977 (2016).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Théry, C. et al. Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines. J. Extracell. Vesicles 7, 1535750 (2018).

Article  PubMed  PubMed Central 

Comments (0)

No login
gif