ZJQ-3F regulates synaptic dysfunction in the cortex and hippocampus of APP/PS1/Tau transgenic mice. (A) The expression of SYP and PSD95 in the cortex (temporal cortex) in APP/PS1/Tau mice. Immunostaining for PSD95 (red), SYP (green), DAPI (blue) and Merge (red + green + blue) in the cortex (100 µm). (B-C) Quantification of the fluorescence intensity of PSD95 and SYP from the cortex (n = 3). Data were presented as mean ± SEM; One-way ANOVA followed by Tukey’s post-hoc multiple comparisons test; #p < 0.05, ##p < 0.01 vs the APP/PS1/Tau group. (D) Representative immunoblots of PSD95, SYP, SYT and GAPDH in the cortex of APP/PS1/Tau mice. (E-G) Quantification of PSD95, SYP and SYT in the cortex of APP/PS1/Tau mice (n = 6). Data were presented as mean ± SEM; One-way ANOVA followed by Tukey’s post-hoc multiple comparisons test; *p < 0.05 vs the WT group; #p < 0.05, ##p < 0.01, ###p < 0.001 vs the APP/PS1/Tau group. (H) The expression of SYP and PSD95 in the hippocampus in APP/PS1/Tau mice. Immunostaining for PSD95 (red), SYP (green), DAPI (blue) and Merge (red + green + blue) in the hippocampus (500 µm). (I-J) Quantification of the fluorescence intensity of PSD95 and SYP from the hippocampus (n = 3). Data were presented as mean ± SEM; One-way ANOVA followed by Tukey’s post-hoc multiple comparisons test; #p < 0.05vs the APP/PS1/Tau group. (K) Representative immunoblots of PSD95, SYP, SYT and GAPDH in the hippocampus of APP/PS1/Tau mice. (L-N) Quantification of PSD95, SYP and SYT in the hippocampus of APP/PS1/Tau mice (n = 6). Data were presented as mean ± SEM; One-way ANOVA followed by Tukey’s post-hoc multiple comparisons test; *p < 0.05, ***p < 0.001 vs the WT group; ##p < 0.01, ###p < 0.001 vs the APP/PS1/Tau group