A novel tape-free sample preparation method for human osteochondral cryosections for high throughput hyperspectral imaging

Amenabar I, Poly S, Goikoetxea M, Nuansing W, Lasch P, Hillenbrand R (2017) Hyperspectral infrared nanoimaging of organic samples based on Fourier transform infrared nanospectroscopy. Nat Commun 8:14402. https://doi.org/10.1038/ncomms14402

Article  CAS  PubMed  PubMed Central  Google Scholar 

Burkhart KJ, Nowak TE, Blum J, Kuhn S, Welker M, Sternstein W, Mueller LP, Rommens PM (2010) Influence of formalin fixation on the biomechanical properties of human diaphyseal bone. Biomed Tech (Berl) 55(6):361–365. https://doi.org/10.1515/bmt.2010.043

Article  PubMed  Google Scholar 

Ciani A, Toumi H, Pallu S, Tsai EHR, Diaz A, Guizar-Sicairos M, Holler M, Lespessailles E, Kewish CM (2018) Ptychographic X-ray CT characterization of the osteocyte lacuno-canalicular network in a male rat’s glucocorticoid induced osteoporosis model. Bone Rep 9:122–131. https://doi.org/10.1016/j.bonr.2018.07.005

Article  PubMed  PubMed Central  Google Scholar 

Cometta S, Jones RT, Juárez-Saldivar A, Donose BC, Yasir M, Bock N, Dargaville TR, Bertling K, Brünig M, Rakić AD, Willcox M, Hutmacher DW (2022) Melimine-modified 3D-printed polycaprolactone scaffolds for the prevention of biofilm-related biomaterial infections. ACS Nano 16(10):16497–16512. https://doi.org/10.1021/acsnano.2c05812

Article  CAS  PubMed  PubMed Central  Google Scholar 

Deng J, Vine DJ, Chen S, Nashed YS, Jin Q, Phillips NW, Peterka T, Ross R, Vogt S, Jacobsen CJ (2015) Simultaneous cryo X-ray ptychographic and fluorescence microscopy of green algae. Proc Natl Acad Sci U S A 112(8):2314–2319. https://doi.org/10.1073/pnas.1413003112

Article  CAS  PubMed  PubMed Central  Google Scholar 

Di Luca A, Van Blitterswijk C, Moroni L (2015) The osteochondral interface as a gradient tissue: from development to the fabrication of gradient scaffolds for regenerative medicine. Birth Defects Res C Embryo Today 105(1):34–52. https://doi.org/10.1002/bdrc.21092

Article  CAS  PubMed  Google Scholar 

Fan X, Wu X, Crawford R, Xiao Y, Prasadam I (2021) Macro, micro, and molecular. changes of the osteochondral interface in osteoarthritis development. Front Cell Dev Biol 9:659654. https://doi.org/10.3389/fcell.2021.659654

Article  PubMed  PubMed Central  Google Scholar 

Fan X, Lee KM, Jones MWM, Howard D, Crawford R, Prasadam I (2022a) A technique for preparing undecalcified osteochondral fresh frozen sections for elemental mapping and understanding disease etiology. Histochem Cell Biol 158(5):463–469. https://doi.org/10.1007/s00418-022-02135-8

Article  CAS  PubMed  PubMed Central  Google Scholar 

Fan X, Wu X, Lima TFD, L, Stehbens S, Punyadeera C, Webb R, Hamilton B, Ayyapann V, McLauchlan C, Crawford R, Zheng M, Xiao Y, Prasadam I, (2022b) The deterioration of calcified cartilage integrity reflects the severity of osteoarthritis-A structural, molecular, and biochemical analysis. Faseb j 36(2):e22142. https://doi.org/10.1096/fj.202101449R

Article  CAS  PubMed  Google Scholar 

Fan X, Lee KM, Jones MWM, Howard D, Sun AR, Crawford R, Prasadam I (2023) Spatial distribution of elements during osteoarthritis disease progression using synchrotron X-ray fluorescence microscopy. Sci Rep 13(1):10200. https://doi.org/10.1038/s41598-023-36911-w

Article  CAS  PubMed  PubMed Central  Google Scholar 

Fang M, Goldstein EL, Turner AS, Les CM, Orr BG, Fisher GJ, Welch KB, Rothman ED, Banaszak Holl MM (2012) Type I collagen D-spacing in fibril bundles of dermis, tendon, and bone: bridging between nano- and micro-level tissue hierarchy. ACS Nano 6(11):9503–9514. https://doi.org/10.1021/nn302483x

Article  CAS  PubMed  PubMed Central  Google Scholar 

Furman BD, Strand J, Hembree WC, Ward BD, Guilak F, Olson SA (2007) Joint degeneration following closed intraarticular fracture in the mouse knee: a model of posttraumatic arthritis. J Orthop Res 25(5):578–592. https://doi.org/10.1002/jor.20331

Article  PubMed  Google Scholar 

Garcia R, Proksch R (2013) Nanomechanical mapping of soft matter by bimodal force microscopy. Eur Polymer J 49(8):1897–1906. https://doi.org/10.1016/j.eurpolymj.2013.03.037

Article  CAS  Google Scholar 

Genoud S, Jones MWM, Trist BG, Deng J, Chen S, Hare DJ, Double KL (2020) Simultaneous structural and elemental nano-imaging of human brain tissue. Chem Sci 11(33):8919–8927. https://doi.org/10.1039/d0sc02844d

Article  CAS  PubMed  PubMed Central  Google Scholar 

Goldring SR, Goldring MB (2016) Changes in the osteochondral unit during osteoarthritis: structure, function and cartilage-bone crosstalk. Nat Rev Rheumatol 12(11):632–644. https://doi.org/10.1038/nrrheum.2016.148

Article  PubMed  Google Scholar 

Guo X, Degnan Z, Steele JA, Solano E, Donose BC, Bertling K, Fedorov A, Rakić AD, Jacobson P (2023) Near-Field Localization of the Boson Peak on Tantalum Films for Superconducting Quantum Devices. J Phys Chem Lett 14(20):4892–4900. https://doi.org/10.1021/acs.jpclett.3c00850

Article  CAS  PubMed  Google Scholar 

Hackett MJ, McQuillan JA, El-Assaad F, Aitken JB, Levina A, Cohen DD, Siegele R, Carter EA, Grau GE, Hunt NH, Lay PA (2011) Chemical alterations to murine brain tissue induced by formalin fixation: implications for biospectroscopic imaging and mapping studies of disease pathogenesis. Analyst 136(14):2941–2952. https://doi.org/10.1039/c0an00269k

Article  CAS  PubMed  Google Scholar 

Howard DL, de Jonge MD, Afshar N, Ryan CG, Kirkham R, Reinhardt J, Kewish CM, McKinlay J, Walsh A, Divitcos J, Basten N, Adamson L, Fiala T, Sammut L, Paterson DJ (2020) The XFM beamline at the Australian Synchrotron. J Synchrotron Radiat 27(Pt 5):1447–1458. https://doi.org/10.1107/s1600577520010152

Article  CAS  PubMed  Google Scholar 

Huang W, Shishehbor M, Guarín-Zapata N, Kirchhofer ND, Li J, Cruz L, Wang T, Bhowmick S, Stauffer D, Manimunda P, Bozhilov KN, Caldwell R, Zavattieri P, Kisailus D (2020) A natural impact-resistant bicontinuous composite nanoparticle coating. Nat Mater 19(11):1236–1243. https://doi.org/10.1038/s41563-020-0768-7

Article  CAS  PubMed  Google Scholar 

Huth F, Govyadinov A, Amarie S, Nuansing W, Keilmann F, Hillenbrand R (2012) Nano-FTIR absorption spectroscopy of molecular fingerprints at 20 nm spatial resolution. Nano Lett 12(8):3973–3978. https://doi.org/10.1021/nl301159v

Article  CAS  PubMed  Google Scholar 

Jin Q, Paunesku T, Lai B, Gleber SC, Chen SI, Finney L, Vine D, Vogt S, Woloschak G, Jacobsen C (2017) Preserving elemental content in adherent mammalian cells for analysis by synchrotron-based x-ray fluorescence microscopy. J Microsc 265(1):81–93. https://doi.org/10.1111/jmi.12466

Article  CAS  PubMed  Google Scholar 

Jones MW, Phillips NW, Van Riessen GA, Abbey B, Vine DJ, Nashed YS, Mudie ST, Afshar N, Kirkham R, Chen B (2016) Simultaneous X-ray fluorescence and scanning X-ray diffraction microscopy at the Australian Synchrotron XFM beamline. J Synchrotron Radiat 23(5):1151–1157

Article  PubMed  Google Scholar 

Jones MWM, Hare DJ, James SA, de Jonge MD, McColl G (2017) Radiation dose limits for bioanalytical X-ray fluorescence microscopy. Anal Chem 89(22):12168–12175. https://doi.org/10.1021/acs.analchem.7b02817

Article  CAS  PubMed  Google Scholar 

Jones MWM, Phillips NW, Abbey B, Hare DJ, van Riessen GA, Vine DJ, de Jonge MD, McColl G (2019) Simultaneous nanostructure and chemical imaging of intact whole nematodes. Chem Commun (Camb) 55(8):1052–1055. https://doi.org/10.1039/c8cc09664c

Article  CAS  PubMed  Google Scholar 

Jones MW, van Riessen GA, Phillips NW, Schrank CE, Hinsley GN, Afshar N, Reinhardt J, de Jonge MD, Kewish CM (2022) High-speed free-run ptychography at the Australian Synchrotron. J Synchrotron Radiat 29(2):480–487

Article  PubMed  PubMed Central  Google Scholar 

Kawamoto T, Kawamoto K (2021) Preparation of thin frozen sections from nonfixed and undecalcified hard tissues using kawamoto’s film method (2020). Methods Mol Biol 2230:259–281. https://doi.org/10.1007/978-1-0716-1028-2_15

Article  CAS  PubMed  Google Scholar 

Mester L, Govyadinov AA, Chen S, Goikoetxea M, Hillenbrand R (2020) Subsurface chemical nanoidentification by nano-FTIR spectroscopy. Nat Commun 11(1):3359.

Comments (0)

No login
gif