Exercise affects the immune function and induces pro- and anti-inflammatory effects. The alterations concerning the immune system linked to physical activity have been documented across various studies with complex exercise tests. However, the characterization of the non-pathological, exercise-induced immunological stress regulation is highly relevant in numerous clinical and non-clinical areas for a better understanding of normal physiological adaptations and differentiation from non-healthy adaptations. Thus, it is valuable and necessary to establish simple immune-metabolic response triggering exercise tests for use in clinical and non-clinical settings. The aim of this study was to examine the effects of the 1-minute sit-to-stand test (STST) on immune-metabolic stress indices and to determine whether it elicits a sufficiently high intensity to qualify as an anaerobic exercise test, thereby supporting its application in investigating exercise-induced immunological stress regulation. 28 study participants performed the 1-minute STST. Capillary blood was taken 20 and 10 minutes before the test, immediately after, and 5, 10, 15, 30, and 45 minutes after exercise. Lactate, glucose and blood counts were determined. Lactate concentration increased significantly immediately after the STST (p < 0.001) and remained significantly elevated until 45 minutes post-exercise. Glucose concentration was significantly decreased after 10 minutes post-exercise (p < 0.05) and again 30 and 45 minutes post-exercise (p < 0.01 for both). Leucocyte count increased significantly post-exercise (p < 0.001) and returned to baseline levels 30 minutes afterwards. Lymphocyte and granulocyte count increased significantly after the test (p < 0.001 for both) and lymphocyte count slightly decreased below baseline values 30 minutes post-exercise (p = 0.07) while granulocyte count remained significantly elevated (p < 0.05). Furthermore, decreased NLR (p < 0.001) and SII (p < 0.01) immediately after the test and increased levels of NLR, SII and SIRI post-exercise could be observed. The 1-minute STST caused an increase in lactate level above the anaerobic threshold, therefore the test can be evaluated as an anaerobic exercise test. Furthermore, it was demonstrated that the STST induced shifts in leucocyte, lymphocyte, and granulocyte counts, which means that even a short intense anaerobic exercise, such as the STST causes changes in immune cell counts and therefore, the test is suitable for analyzing the exercise-induced immunological stress response.

The authors have declared no competing interest.

This research was supported by the German Federal Institute of Sport Science (ZMVI4-081901/20-23) and the Federal Ministry of Research, Technology and Space (01EJ2408A). All analyses were performed as part of the project 01EJ2408A.

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The Protocol was approved by the University Ethics Committee of the Friedrich Schiller University/University Hospital Jena (458510/15, 2025-3763-BO-A).

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