Measuring Neural Factor X Pathway Activity in Rodent Glioma Cell Culture and Brain Slices

Gofrit SG, Shavit-Stein E (2019) The neuro-glial coagulonome: the thrombin receptor and coagulation pathways as major players in neurological diseases. Neural Regen Res 14:2043–2053

Article  PubMed  PubMed Central  Google Scholar 

Shavit-Stein E, Aronovich R, Sylantiev C et al (2019) Blocking thrombin significantly ameliorates experimental autoimmune neuritis. Front Neurol. https://doi.org/10.3389/fneur.2018.01139

Article  PubMed  PubMed Central  Google Scholar 

Itsekson-Hayosh Z, Shavit-Stein E, Last D et al (2015) Thrombin activity and thrombin receptor in rat glioblastoma model: possible markers and targets for intervention? J Mol Neurosci 56:644–651

Article  PubMed  CAS  Google Scholar 

Troy GC (1988) An overview of hemostasis. Vet Clin North Am Small Anim Pract 18:5–20. https://doi.org/10.1016/S0195-5616(88)50003-7

Article  PubMed  CAS  Google Scholar 

Bushi D, Chapman J, Wohl A et al (2018) Apixaban decreases brain thrombin activity in a male mouse model of acute ischemic stroke. J Neurosci Res 96:1406–1411

Article  PubMed  CAS  Google Scholar 

Gera O, Bushi D, Ben Shimon M et al (2018) Local regulation of thrombin activity by factor Xa in peripheral nerve Schwann cells. Neuroscience 371:445–454

Article  PubMed  CAS  Google Scholar 

Shavit-Stein E, Gofrit SG, Gayster A et al (2020) Treatment of diabetic neuropathy with a novel PAR1-targeting molecule. Biomolecules 10:1552

Article  PubMed  PubMed Central  CAS  Google Scholar 

Golderman V, Goldberg Z, Gofrit SG et al (2023) PARIN5, a novel thrombin receptor antagonist modulates a streptozotocin mice model for diabetic encephalopathy. Int J Mol Sci 24:2021

Article  PubMed  PubMed Central  CAS  Google Scholar 

Golderman V, Ben-Shimon M, Maggio N et al (2022) Factor VII, EPCR, aPC modulators: novel treatment for neuroinflammation. J Neuroinflammation 19:138

Article  PubMed Central  CAS  Google Scholar 

Bushi D, Gera O, Kostenich G et al (2016) A novel histochemical method for the visualization of thrombin activity in the nervous system. Neuroscience 320:93–104

Article  CAS  Google Scholar 

Golderman V, Gofrit SG, Maggio N et al (2020) A novel highly sensitive method for measuring inflammatory neural-derived APC activity in glial cell lines, mouse brain and human CSF. Int J Mol Sci 21:2422

Article  PubMed Central  CAS  Google Scholar 

Bushi D, Chapman J, Katzav A et al (2013) Quantitative detection of thrombin activity in an ischemic stroke model. J Mol Neurosci 51:844–850

Article  CAS  Google Scholar 

Bowman RL, Wang Q, Carro A et al (2017) Gliovis data portal for visualization and analysis of brain tumor expression datasets. Neuro-Oncology 19:139–141

Article  PubMed  CAS  Google Scholar 

Shavit-Stein E, Sheinberg E, Golderman V et al (2018) A novel compound targeting protease receptor 1 activators for the treatment of glioblastoma. Front Neurol. https://doi.org/10.3389/fneur.2018.01087

Article  PubMed Central  Google Scholar 

Schuepbach RA, Riewald M (2010) Coagulation factor Xa cleaves protease-activated receptor-1 and mediates signaling dependent on binding to the endothelial protein C receptor. J Thromb Haemost 8:379–388

Article  PubMed  CAS  Google Scholar 

Sloan AR, Lee-Poturalski C, Hoffman HC et al (2022) Glioma stem cells activate platelets by plasma-independent thrombin production to promote glioblastoma tumorigenesis. Neuro-Oncol Adv 4:vdac172

Article  Google Scholar 

Golderman V, Gofrit SG, Ivashko-Pachima Y et al (2024) The thrombin receptor (PAR1) is associated with microtubules, mitosis and process formation in glioma cells. Heliyon. https://doi.org/10.1016/j.heliyon.2024.e33329

Article  PubMed  PubMed Central  Google Scholar 

Chevreux G, Tilly N, Faid V, Bihoreau N (2015) Mass spectrometry based analysis of human plasma-derived factor X revealed novel post-translational modifications. Protein Sci 24:1640–1648

Article  PubMed Central  CAS  Google Scholar 

de Vries JJ, Snoek CJM, Rijken DC, de Maat MPM (2020) Effects of post-translational modifications of fibrinogen on clot formation, clot structure, and fibrinolysis. Arterioscler Thromb Vasc Biol 40:554–569

Article  PubMed  Google Scholar 

Kalafatis M, Rand M, Jenny R et al (1993) Phosphorylation of factor Va and factor VIIIa by activated platelets. Blood 81:704–719

Article  CAS  Google Scholar 

Mann K, Nesheim M, Church W et al (1990) Surface-dependent reactions of the vitamin K-dependent enzyme complexes. Blood 76:1–16

Article  PubMed  CAS  Google Scholar 

Wood JP, Petersen HH, Yu B et al (2017) TFPIα interacts with FVa and FXa to inhibit prothrombinase during the initiation of coagulation. Blood Adv 1:2692–2702

Article  PubMed  PubMed Central  CAS  Google Scholar 

Ruf W (2021) Roles of factor Xa beyond coagulation. J Thromb Thrombolysis 52:391–396

Article  PubMed  PubMed Central  CAS  Google Scholar 

Green D (2006) Coagulation cascade. Hemodial Int 10:S2–S4

Article  Google Scholar 

Mladěnka P, Macáková K, Kujovská Krčmová L et al (2022) Vitamin K – sources, physiological role, kinetics, deficiency, detection, therapeutic use, and toxicity. Nutr Rev 80:677–698

Article  PubMed  Google Scholar 

Hua Y, Tang L, Keep RF et al (2005) The role of thrombin in gliomas. J Thromb Haemost 3:1917–1923

Article  PubMed  CAS  Google Scholar 

Hua Y, Tang L, Keep RF et al (2009) Thrombin enhances glioma growth. In: Steiger H-J (ed) Acta Neurochirurgica Supplements. Springer, Vienna, pp 363–366

Google Scholar 

Sakellaridis N, Mangoura D, Vernadakis A (1984) Glial cell growth in culture: influence of living cell substrata. Neurochem Res 9:1477–1491

Article  PubMed  CAS  Google Scholar 

Norström E, Escolar G (2011) Natural anticoagulants and thrombophilia. Blood and Bone Marrow Pathology. Churchill Livingstone, pp 583–595

Chapter  Google Scholar 

Wood JP, Ellery PER, Maroney SA, Mast AE (2014) Biology of tissue factor pathway inhibitor. Blood 123:2934–2943

Article  PubMed  PubMed Central  CAS  Google Scholar 

Verhoef D, Tjalma AVR, Cheung KL et al (2021) Elevated anti-human factor Xa activity in rabbit and rodent plasma: implications for preclinical assessment of human factor X in animal models of hemostasis. Thromb Res 198:154–162

Article  PubMed  CAS  Google Scholar 

Jacobs VL, Valdes PA, Hickey WF, De Leo JA (2011) Current review of in vivo GBM rodent models: emphasis on the CNS-1 tumour model. ASN Neuro 3:e00063

Article  PubMed  PubMed Central  Google Scholar 

Opal SM (2000) Phylogenetic and functional relationships between coagulation and the innate immune response. Crit Care Med 28:S77

Article  CAS  Google Scholar 

Loof TG, Mörgelin M, Johansson L et al (2011) Coagulation, an ancestral serine protease cascade, exerts a novel function in early immune defense. Blood 118:2589–2598

Comments (0)

No login
gif