Abud EM, Ramirez RN, Martinez ES, Healy LM, Nguyen CH, Newman SA, Yeromin AV, Scarfone VM, Marsh SE, Fimbres C (2017) iPSC-derived human microglia-like cells to study neurological diseases. Neuron 94:278-293. e279 https://doi.org/10.1016/j.neuron.2017.03.042

Article  PubMed  PubMed Central  Google Scholar 

Au NPB, Ma CHE (2022) Neuroinflammation, microglia and implications for retinal ganglion cell survival and axon regeneration in traumatic optic neuropathy. Front Immunol 13:860070 https://doi.org/10.3389/fimmu.2022.860070

Article  PubMed  PubMed Central  Google Scholar 

Bartalska K, Hübschmann V, Korkut-Demirbaş M, Cubero RJA, Venturino A, Rössler K, Czech T, Siegert S (2022) A systematic characterization of microglia-like cell occurrence during retinal organoid differentiation. iScience. https://doi.org/10.1016/j.isci.2022.104580

Article  PubMed  PubMed Central  Google Scholar 

Baxter PS, Dando O, Emelianova K, He X, McKay S, Hardingham GE, Qiu J (2021) Microglial identity and inflammatory responses are controlled by the combined effects of neurons and astrocytes. Cell Rep. https://doi.org/10.1016/j.celrep.2021.108882

Article  PubMed  PubMed Central  Google Scholar 

Buchrieser J, James W, Moore MD (2017) Human induced pluripotent stem cell-derived macrophages share ontogeny with MYB-independent tissue-resident macrophages. Stem Cell Rep 8:334–345 https://doi.org/10.1016/j.stemcr.2016.12.020

Article  Google Scholar 

Cadiz MP, Jensen TD, Sens JP, Zhu K, Song W-M, Zhang B, Ebbert M, Chang R, Fryer JD (2022) Culture shock: microglial heterogeneity, activation, and disrupted single-cell microglial networks in vitro. Mol Neurodegener 17:26 https://doi.org/10.1186/s13024-022-00531-1

Article  PubMed  PubMed Central  Google Scholar 

Cardona AE, Pioro EP, Sasse ME, Kostenko V, Cardona SM, Dijkstra IM, Huang D, Kidd G, Dombrowski S, Dutta R, Lee JC, Cook DN, Jung S, Lira SA, Littman DR, Ransohoff RM (2006) Control of microglial neurotoxicity by the fractalkine receptor. Nat Neurosci 9:917–924 https://doi.org/10.1038/nn1715

Article  PubMed  Google Scholar 

Chadarevian JP, Davtyan H, Chadarevian AL, Nguyen J, Capocchi JK, Le L, Escobar A, Chadarevian T, Mansour K, Deynega E (2025) Harnessing human iPSC-microglia for CNS-wide delivery of disease-modifying proteins. Cell Stem Cell32(6):914-934.e8. https://doi.org/10.1016/j.stem.2025.03.009

Article  PubMed  Google Scholar 

Chen S-W, Hung Y-S, Fuh J-L, Chen N-J, Chu Y-S, Chen S-C, Fann M-J, Wong Y-H (2021) Efficient conversion of human induced pluripotent stem cells into microglia by defined transcription factors. Stem Cell Rep 16:1363–1380 https://doi.org/10.1016/j.stemcr.2021.03.010

Article  Google Scholar 

Chen WW, Wu Z, Li H (2025) The microglial lineage: present and beyond. Trends Immunol46(6):438-440. https://doi.org/10.1016/j.it.2025.04.001

Article  PubMed  Google Scholar 

Chichagova V, Georgiou M, Carter M, Dorgau B, Hilgen G, Collin J, Queen R, Chung G, Ajeian J, Moya-Molina M, Kustermann S, Pognan F, Hewitt P, Schmitt M, Sernagor E, Armstrong L, Lako M (2023) Incorporating microglia-like cells in human induced pluripotent stem cell-derived retinal organoids. J Cell Mol Med 27:435–445 https://doi.org/10.1111/jcmm.17670

Damani MR, Zhao L, Fontainhas AM, Amaral J, Fariss RN, Wong WT (2011) Age-related alterations in the dynamic behavior of microglia. Aging Cell 10:263–276 https://doi.org/10.1111/j.1474-9726.2010.00660.x

Article  PubMed  Google Scholar 

Deng Y, Qiao L, Du M, Qu C, Wan L, Li J, Huang L (2022) Age-related macular degeneration: epidemiology, genetics, pathophysiology, diagnosis, and targeted therapy. Genes Dis 9:62–79 https://doi.org/10.1016/j.gendis.2021.02.009

Article  PubMed  Google Scholar 

Ding X, Wang J, Huang M, Chen Z, Liu J, Zhang Q, Zhang C, Xiang Y, Zen K, Li L (2021) Loss of microglial SIRPα promotes synaptic pruning in preclinical models of neurodegeneration. Nat Commun 12 https://doi.org/10.1038/s41467-021-22301-1

Article  PubMed  PubMed Central  Google Scholar 

Dolan MJ, Therrien M, Jereb S, Kamath T, Gazestani V, Atkeson T, Marsh SE, Goeva A, Lojek NM, Murphy S, White CM, Joung J, Liu B, Limone F, Eggan K, Hacohen N, Bernstein BE, Glass CK, Leinonen V, Blurton-Jones M, Zhang F, Epstein CB, Macosko EZ, Stevens B (2023) Exposure of iPSC-derived human microglia to brain substrates enables the generation and manipulation of diverse transcriptional states in vitro. Nat Immunol 24:1382–1390 https://doi.org/10.1038/s41590-023-01558-2

Article  PubMed  PubMed Central  Google Scholar 

Douvaras P, Buenaventura DF, Sun B, Lepack A, Baker E, Simpson E, Ebel M, Lallos G, LoSchiavo D, Stitt N (2024) Ready-to-use iPSC-derived microglia progenitors for the treatment of CNS disease in mouse models of neuropathic mucopolysaccharidoses. Nat Commun 15 https://doi.org/10.1038/s41467-024-52400-8

Article  PubMed  PubMed Central  Google Scholar 

Dräger NM, Sattler SM, Huang CT-L, Teter OM, Leng K, Hashemi SH, Hong J, Aviles G, Clelland CD, Zhan L (2022) A CRISPRi/a platform in human iPSC-derived microglia uncovers regulators of disease states. Nat Neurosci 25:1149–1162 https://doi.org/10.1038/s41593-022-01131-4

Article  PubMed  PubMed Central  Google Scholar 

Eiraku M, Takata N, Ishibashi H, Kawada M, Sakakura E, Okuda S, Sekiguchi K, Adachi T, Sasai Y (2011) Self-organizing optic-cup morphogenesis in three-dimensional culture. Nature 472:51–56 https://doi.org/10.1038/nature09941

Article  PubMed  Google Scholar 

Fantin A, Vieira JM, Gestri G, Denti L, Schwarz Q, Prykhozhij S, Peri F, Wilson SW, Ruhrberg C (2010) Tissue macrophages act as cellular chaperones for vascular anastomosis downstream of VEGF-mediated endothelial tip cell induction. Blood 116:829–840 https://doi.org/10.1182/blood-2009-12-257832

Article  PubMed  PubMed Central  Google Scholar 

Fletcher EL (2020) Contribution of microglia and monocytes to the development and progression of age related macular degeneration. Ophthalmic Physiol Opt 40:128–139 https://doi.org/10.1111/opo.12671

Funes S, Jung J, Gadd DH, Mosqueda M, Zhong J, Shankaracharya, Unger M, Stallworth K, Cameron D, Rotunno MS (2024) Expression of ALS-PFN1 impairs vesicular degradation in iPSC-derived microglia. Nat Commun 15:2497 https://doi.org/10.1038/s41467-024-46695-w

Article  PubMed  PubMed Central  Google Scholar 

Gao M-L, Zhang X, Han F, Xu J, Yu S-J, Jin K, Jin Z-B (2022) Functional microglia derived from human pluripotent stem cells empower retinal organ. Sci China Life Sci 65:1057–1071 https://doi.org/10.1007/s11427-021-2086-0

Article  PubMed  Google Scholar 

Gao M-L, Wang T-Y, Lin X, Tang C, Li M, Bai Z-P, Liu Z-C, Chen L-J, Kong Q-R, Pan S-H (2024) Retinal organoid microenvironment enhanced bioactivities of Microglia-Like cells derived from HiPSCs. Invest Ophthalmol Vis Sci 65:19–19 https://doi.org/10.1167/iovs.65.12.19

Article  PubMed  PubMed Central  Google Scholar 

Ginhoux F, Greter M, Leboeuf M, Nandi S, See P, Gokhan S, Mehler MF, Conway SJ, Ng LG, Stanley ER, Samokhvalov IM, Merad M (2010) Fate mapping analysis reveals that adult microglia derive from primitive macrophages. Science 330:841–845 https://doi.org/10.1126/science.1194637

Article  PubMed  PubMed Central  Google Scholar 

Goshi N, Morgan RK, Lein PJ, Seker E (2020) A primary neural cell culture model to study neuron, astrocyte, and microglia interactions in neuroinflammation. J Neuroinflamm 17:1–16 https://doi.org/10.1186/s12974-020-01819-z

Article  Google Scholar 

Gosselin D, Skola D, Coufal NG, Holtman IR, Schlachetzki JC, Sajti E, Jaeger BN, O’Connor C, Fitzpatrick C, Pasillas MP (2017) An environment-dependent transcriptional network specifies human microglia identity. Science 356 https://doi.org/10.1126/science.aal3222

Article  PubMed  PubMed Central  Google Scholar 

Gunner G, Cheadle L, Johnson KM, Ayata P, Badimon A, Mondo E, Nagy MA, Liu L, Bemiller SM, Kim KW, Lira SA, Lamb BT, Tapper AR, Ransohoff RM, Greenberg ME, Schaefer A, Schafer DP (2019) Sensory lesioning induces microglial synapse elimination via ADAM10 and fractalkine signaling. Nat Neurosci 22:1075–1088 https://doi.org/10.1038/s41593-019-0419-y

Article  PubMed  PubMed Central  Google Scholar 

Guttikonda SR, Sikkema L, Tchieu J, Saurat N, Walsh RM, Harschnitz O, Ciceri G, Sneeboer M, Mazutis L, Setty M (2021) Fully defined human pluripotent stem cell-derived microglia and tri-culture system model C3 production in alzheimer’s disease. Nat Neurosci 24:343–354 https://doi.org/10.1038/s41593-020-00796-z

Article  PubMed  PubMed Central  Google Scholar 

Haenseler W, Sansom SN, Buchrieser J, Newey SE, Moore CS, Nicholls FJ, Chintawar S, Schnell C, Antel JP, Allen ND (2017) A highly efficient human pluripotent stem cell microglia model displays a neuronal-co-culture-specific expression profile and inflammatory response. Stem Cell Rep 8:1727–1742 https://doi.org/10.1016/j.stemcr.2017.05.017

Article  Google Scholar 

Haines JL, Hauser MA, Schmidt S, Scott WK, Olson LM, Gallins P, Spencer KL, Kwan SY, Noureddine M, Gilbert JR, Schnetz-Boutaud N, Agarwal A, Postel EA, Pericak-Vance MA (2005) Complement factor H variant increases the risk of age-related macular degeneration. Science 308:419–421 https://doi.org/10.1126/science.1109557