Antimicrobial Peptide -Crustin as a Therapeutic Agent against WSSV Infection in Culture System, Conferring Better Protection and Survival

Adachi K, Hirata T, Nishioka T, Sakaguchi M (2003) Hemocyte components in crustaceans convert hemocyanin into a phenoloxidase-like enzyme. Comp Biochem Physiol B: Biochem Mol Biol 134:135–141. https://doi.org/10.1016/s1096-4959(02)00220-8

Article  PubMed  Google Scholar 

Apha Awwa WPCF (2005) Standard methods for the examination of water and wastewater. APHA WEF AWWA

Arts JA (2006) Immune defence of white spot syndrome Virus-infected shrimp. Penaeus Monodon

Aguirre-Guzman G, Sanchez-Martinez JG, Campa-Cordova AI, Luna-Gonzalez A, Ascencio F (2009) Penaeid shrimp immune system. Thai J Vet Med 39:205–215. https://doi.org/10.56808/2985-1130.2175

Article  Google Scholar 

Amparyup P, Donpudsa S, Tassanakajon A (2008) Shrimp single WAP domain (SWD)-containing protein exhibits proteinase inhibitory and antimicrobial activities. Dev Comp Immunol 32:1497–1509. https://doi.org/10.1016/j.dci.2008.06.005

Article  CAS  PubMed  Google Scholar 

Anoop BS, Puthumana J, Vazhappilly CG, Kombiyil S, Philip R, Abdulaziz A, Singh ISB (2021) Immortalization of shrimp lymphoid cells by hybridizing with the continuous cell line Sf9 leading to the development of ‘PmLyO-Sf9’. Fish Shellfish Immunol 113:196–207. https://doi.org/10.1016/j.fsi.2021.03.023

Article  CAS  PubMed  Google Scholar 

Antony SP, Philip R, Joseph V, Singh IB (2011a) Anti-lipopolysaccharide factor and crustin-III, the anti-white spot virus peptides in Penaeus monodon: control of viral infection by up-regulation. Aquaculture 319:11–17. https://doi.org/10.1016/j.aquaculture.2011.06.022

Article  CAS  Google Scholar 

Antony SP, Singh IB, Jose RM, Kumar PA, Philip R (2011b) Antimicrobial peptide gene expression in tiger shrimp, Penaeus monodon, in response to gram-positive bacterial probionts and white spot virus challenge. Aquaculture 316:6–12. https://doi.org/10.1016/j.aquaculture.2011.03.025

Article  CAS  Google Scholar 

Antony SP, Singh IB, Sudheer NS, Vrinda S, Priyaja P, Philip R (2011c) Molecular characterization of a crustin-like antimicrobial peptide in the giant tiger shrimp, Penaeus monodon, and its expression profile in response to various immunostimulants and challenge with WSSV. Immunobiology 216:184–194. https://doi.org/10.1016/j.imbio.2010.05.030

Article  CAS  PubMed  Google Scholar 

Apu AS, Muhit MA, Tareq SM, Pathan AH, Jamaluddin ATM, Ahmed M (2010) Antimicrobial activity and brine shrimp lethality bioassay of the leaves extract of Dillenia indica Linn. J Young Pharm 2:50–53. https://doi.org/10.4103/0975-1483.62213

Article  PubMed  PubMed Central  Google Scholar 

Arockiaraj J, Gnanam AJ, Muthukrishnan D, Gudimella R, Milton J, Singh A, Muthupandian S, Kasi M, Bhassu S (2013) Crustin, a WAP domain containing antimicrobial peptide from freshwater prawn Macrobrachium rosenbergii: immune characterization. Fish Shellfish Immunol 34:109–118. https://doi.org/10.1016/j.fsi.2012.10.009

Article  CAS  PubMed  Google Scholar 

Baird S, Kelly SM, Price NC, Jaenicke E, Meesters C, Nillius D, Decker H, Nairn J (2007) Hemocyanin conformational changes associated with SDS-induced phenol oxidase activation. Biochim Et Biophys Acta (BBA) - Proteins Proteom 1774:1380–1394. https://doi.org/10.1016/j.bbapap.2007.08.019

Article  CAS  Google Scholar 

Barman D, Nen P, Mandal SC, Kumar V (2013) Immunostimulants for aquaculture health management. J Mar Science: Res Dev 3:1–11. https://doi.org/10.4172/2155-9910.1000134

Article  CAS  Google Scholar 

Barreto C, Coelho JDR, Yuan J, Xiang J, Perazzolo LM, Rosa RD (2018) Specific molecular signatures for type II crustins in Penaeid shrimp uncovered by the identification of crustin-like antimicrobial peptides in Litopenaeus vannamei. Mar Drugs 16(1):31. https://doi.org/10.3390/md16010031

Article  CAS  PubMed  PubMed Central  Google Scholar 

Barreto C, Matos GM, Rosa RD (2022) On the wave of the crustin antimicrobial peptide family: from sequence diversity to function. Fish Shellfish Immunol Rep 3:100069. https://doi.org/10.1016/j.fsirep.2022.100069

Article  PubMed  PubMed Central  Google Scholar 

Boyd CE (1990) Water quality in ponds for aquaculture. Agricultural Experiment Station Series

Le Bloa S, Boidin-Wichlacz C, Cueff-Gauchard V, Rosa RD, Cuvillier-Hot V, Durand L, Methou P, Pradillon F, Cambon-Bonavita MA, Tasiemski A (2020) Antimicrobial peptides and ectosymbiotic relationships: involvement of a novel type IIa Crustin in the life cycle of a deep-sea vent shrimp. Front Immunol 11:1511. https://doi.org/10.3389/fimmu.2020.01511

Article  CAS  PubMed  PubMed Central  Google Scholar 

Cerenius L, Söderhäll K (2004) The prophenoloxidase-activating system in invertebrates. Immunol Rev 198:116–126. https://doi.org/10.1111/j.0105-2896.2004.00116.x

Article  CAS  PubMed  Google Scholar 

Chan W, Shaughnessy AEP, van den Berg CP, Garson MJ, Cheney KL (2021) The validity of brine shrimp (Artemia sp.) toxicity assays to assess the ecological function of marine natural products. J Chem Ecol 47:834–846. https://doi.org/10.1007/s10886-021-01264-z

Article  CAS  PubMed  Google Scholar 

Chand RK, Sahoo PK, Kumari J, Pillai BR, Mishra BK (2006) Dietary administration of bovine lactoferrin influences the immune ability of the giant freshwater prawn Macrobrachium rosenbergii (de Man) and its resistance against Aeromonas hydrophila infection and nitrite stress. Fish Shellfish Immunol 21:119–129. https://doi.org/10.1016/j.fsi.2005.10.010

Article  CAS  PubMed  Google Scholar 

Chen IT, Aoki T, Huang YT, Hirono I, Chen TC, Huang JY, Chang GD, Lo CF, Wang HC (2011) White spot syndrome virus induces metabolic changes resembling the Warburg effect in shrimp hemocytes in the early stage of infection. J Virol 85:12919–12928. https://doi.org/10.1128/JVI.05385-11

Article  CAS  PubMed  PubMed Central  Google Scholar 

Coates CJ, Nairn J (2013) Hemocyanin-derived phenoloxidase activity: a contributing factor to hyperpigmentation in Nephrops norvegicus. Food Chem 140:361–369. https://doi.org/10.1016/j.foodchem.2013.02.072

Article  CAS  PubMed  Google Scholar 

Dai X, Huang X, Zhang Z, Zhang R, Cao X, Zhang C, Wang K, Ren Q (2020) Molecular cloning and expression analysis of two type II crustin genes in the oriental river prawn, Macrobrachium Nipponense. Fish Shellfish Immunol 98:446–456. https://doi.org/10.1016/j.fsi.2020.01.001

Article  PubMed  Google Scholar 

Das S, Mondal K, Haque S (2017) A review on application of probiotic, prebiotic and synbiotic for sustainable development of aquaculture. Growth 14:15

Google Scholar 

Deachamag P, Intaraphad U, Phongdara A, Chotigeat W (2006) Expression of a phagocytosis activating protein (PAP) gene in immunized black tiger shrimp. Aquaculture 255:165–172. https://doi.org/10.1016/j.aquaculture.2006.01.010

Article  CAS  Google Scholar 

Destoumieux-Garzón D, Saulnier D, Garnier J, Jouffrey C, Bulet P, Bachère E (2001) Crustacean immunity: antifungal peptides are generated from the C terminus of shrimp Hemocyanin in response to microbial challenge. J Biol Chem 276:47070–47077. https://doi.org/10.1074/jbc.M103817200

Article  PubMed  Google Scholar 

Du ZQ, Li XC, Wang ZH, Zhao XF, Wang JX (2010) A single WAP domain (SWD)-containing protein with antipathogenic relevance in red swamp crayfish, Procambarus clarkii. Fish Shellfish Immunol 28:134–142. https://doi.org/10.1016/j.fsi.2009.10.009

Article  CAS  PubMed  Google Scholar 

Du ZQ, Li B, Shen XL, Wang K, Du J, Yu XD, Yuan JJ (2019a) A new antimicrobial peptide isoform, Pc-crustin 4 involved in antibacterial innate immune response in freshwater crayfish, Procambarus clarkii. Fish Shellfish Immunol 94:861–870. https://doi.org/10.1016/j.fsi.2019.10.003

Article  CAS  PubMed  Google Scholar 

Du ZQ, Wang Y, Ma HY, Shen XL, Wang K, Du J, Yu XD, Fang WH, Li XC (2019b) A new crustin homologue (SpCrus6) involved in the antimicrobial and antiviral innate immunity in mud crab, Scylla paramamosain. Fish Shellfish Immunol 84:733–743. https://doi.org/10.1016/j.fsi.2018.10.072

Article  CAS  PubMed  Google Scholar 

Durica DS, Kupfer D, Najar F, Lai H, Tang Y, Griffin K, Hopkins PM, Roe B (2006) EST library sequencing of genes expressed during early limb regeneration in the fiddler crab and transcriptional responses to ecdysteroid exposure in limb bud explants. Integr Comp Biol 46:948–964. https://doi.org/10.1093/icb/icl005

Article  CAS  PubMed  Google Scholar 

FAO (2021) The FAO action plan on antimicrobial resistance 2021–2025. Food and Agriculture Organization of the United Nations from https://openknowledge.fao.org/server/api/core/bitstreams/dd6c0ba1-fd85-4a3e-b398-53b610c35318/content

FAO GLOBEFISH (2025) Shrimps – Species analysis. Food and Agriculture Organization of the United Nations. Retrieved June 17, 2025 from

Comments (0)

No login
gif