Paddock LE, Lu SE, Bandera EV, Rhoads GG, Fine J, Paine S, Barnhill R, Berwick M (2016) Skin self-examination and long-term melanoma survival. Melanoma Research 26:401

Article  PubMed  Google Scholar 

Ferlay J, Soerjomataram I, Ervik M, Dikshit R, Eser S, Mathers C, Rebelo M, Parkin DM, Forman D, Bray F in GLOBOCAN 2020, Global Cancer Observatory: cancer today. International Agency for Research on Cancer, Lyon https://gco.iarc.fr/today. Accessed 02 October 2022

Sung H, Ferlay J, Siegel RL, Laversanne M, Soerjomataram I, Jemal A, Bray F (2021) Global Cancer Statistics 2020: GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries. CA A Cancer J Clin 71:209

Article  Google Scholar 

Carr S, Smith C, Wernberg J (2020) Epidemiology and risk factors of melanoma. Surgical Clinics of North Am 100:1

Article  Google Scholar 

O’Day SJ, Hamid O, Urba WJ (2007) Targeting cytotoxic T-lymphocyte antigen-4 (CTLA-4): a novel strategy for the treatment of melanoma and other malignancies. Cancer 110:2614

Article  PubMed  Google Scholar 

Carlino MS, Larkin J, Long GV (2021) immune checkpoint inhibitors in melanoma. Lancet 398:1002

Article  CAS  PubMed  Google Scholar 

Flaherty KT et al (2012) Improved survival with MEK inhibition in BRAF-mutated melanoma. N Engl J Med 367:107

Article  CAS  PubMed  Google Scholar 

Lee J et al (2023) BRAF inhibitor cessation prior to disease progression in metastatic melanoma: long-term outcomes. European Journal of Cancer 179:87

Article  CAS  PubMed  Google Scholar 

Gao Z, Dong K, Zhang H (2014) The roles of CD73 in cancer. BioMed Res Int 2014:1

Google Scholar 

Ferrari D, Malavasi F, Antonioli L (2017) A purinergic trail for metastases. Trends Pharmacol Sci 38:277

Article  CAS  PubMed  Google Scholar 

Di Virgilio F, Sarti AC, Falzoni S, De Marchi E, Adinolfi E (2018) Extracellular ATP and P2 purinergic signalling in the tumour microenvironment. Nat Rev Cancer 18:601

Article  PubMed  Google Scholar 

Mânica A, da Silva Rosa B, Bonadiman AM, Cardoso A, Paiz C, Siepko JVG, de Souza M, Moreno A, Moreno MRC, Schetinger VM, Bagatini MD (2019) The signaling effects of ATP on melanoma-like skin cancer. Cell Signal 59:122

Article  PubMed  Google Scholar 

da Silva GB, Yamauchi MA, Zanini D, Bagatini MD (2022) Novel possibility for cutaneous melanoma treatment by means of rosmarinic acid action on purinergic signaling. Purinergic Signal 18:61

Article  PubMed  Google Scholar 

Pelinson LP et al (2019) Antiproliferative and apoptotic effects of caffeic acid on SK-Mel-28 human melanoma cancer cells. Mol Biol Rep 46:2085

Article  CAS  PubMed  Google Scholar 

Ashihara H, Sano H, Crozier A (2008) Caffeine and related purine alkaloids: biosynthesis, catabolism, function and genetic engineering. Phytochemistry 69:841

Article  CAS  PubMed  Google Scholar 

Miwa S, Sugimoto N, Yamamoto N, Shirai T, Nishida H, Hayashi K, Kimura H, Takeuchi A, Igarashi K, Yachie A, Tsuchiya H (2012) Caffeine induces apoptosis of osteosarcoma cells by inhibiting AKT/mTOR/S6K, NF-κB and MAPK pathways. Anticancer Res 32:3643

CAS  PubMed  Google Scholar 

Wang Z, Zhang L, Wan Z, He Y, Huang H, Xiang H, Wu X, Zhang K, Liu Y, Goodin S, Du Z, Zheng X (2020) Atorvastatin and caffeine in combination regulates apoptosis, migration, invasion and tumorspheres of prostate cancer cells. Pathol Oncol Res 26:209

Article  CAS  PubMed  Google Scholar 

Eini H, Frishman V, Yulzari R, Kachko L, Lewis EC, Chaimovitz C, Douvdevani A (2015) Caffeine promotes anti-tumor immune response during tumor initiation: involvement of the adenosine A2A receptor. Biochem Pharmacol 98:110

Article  CAS  PubMed  Google Scholar 

Wrześniok D, Rzepka Z, Respondek M, Beberok A, Rok J, Szczepanik K, Buszman E (2018) Caffeine modulates growth and vitality of human melanotic COLO829 and amelanotic C32 melanoma cells: preliminary findings. Food Chem Toxicol 120:566

Article  PubMed  Google Scholar 

Mosmann T (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 65:55

Article  CAS  PubMed  Google Scholar 

McGahon AJ, Martin SJ, Bissonnette RP, Mahboubi A, Shi Y, Mogil RJ, Nishioka WK, Green DR (1995) In: Schwartz LM, Osborne BA (eds) Chapter 9 the end of the (cell) line: methods for the study of apoptosis in vitro in methods in cell biology. Academic Press, pp 153–185

Google Scholar 

Joshi DC, Bakowska JC (2011) Determination of mitochondrial membrane potential and reactive oxygen species in live rat cortical neurons. JoVE 2704

Justus CR, Leffler N, Ruiz-Echevarria M, Yang LV (2014) In vitro cell migration and invasion assays. JoVE 51046

Romitelli F, Santini SA, Chierici E, Pitocco D, Tavazzi B, Amorini AM, Lazzarino G, Di Stasio E (2007) Comparison of nitrite/nitrate concentration in human plasma and serum samples measured by the enzymatic batch Griess assay, ion-pairing HPLC and ion-trap GC–MS: the importance of a correct removal of proteins in the Griess assay. J Chromatograph B 851:257

Article  CAS  Google Scholar 

Zahedi Asl S, Ghasemi A, Azizi F (2008) Serum nitric oxide metabolites in subjects with metabolic syndrome. Clin Biochem 41:1342

Article  CAS  PubMed  Google Scholar 

Tatsch E, Bochi GV, da Pereira SR, Kober H, Agertt VA, de Campos MMA, Gomes P, Duarte MMMF, Moresco RN (2011) A simple and inexpensive automated technique for measurement of serum nitrite/nitrate. Clin Biochem 44:348

Article  CAS  PubMed  Google Scholar 

Ellman GL (1959) Tissue sulfhydryl groups. Arch Biochem Biophys 82:70

Article  CAS  PubMed  Google Scholar 

Pilla C, Emanuelli T, Frassetto SS, Battastini AMO, Dias RD, Sarkis JJF (1996) ATP diphosphohydrolase activity (apyrase, EC 3.6.1.5) in human blood platelets. Platelets 7:225

Article  CAS  PubMed  Google Scholar 

Lunkes GI, Lunkes D, Stefanello F, Morsch A, Morsch VM, Mazzanti CM, Schetinger MRC (2003) Enzymes that hydrolyze adenine nucleotides in diabetes and associated pathologies. Thrombosis Res 109:189

Article  CAS  Google Scholar 

Karamohamed S, Guidotti G (2001) Bioluminometric method for real-time detection of ATPase activity. BioTechniques 31:420

Article  CAS  PubMed  Google Scholar 

Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248

Article  CAS  PubMed  Google Scholar 

Miller R, Walker S, Shui I, Brandtmüller A, Cadwell K, Scherrer E (2020) Epidemiology and survival outcomes in stages II and III cutaneous melanoma: a systematic review. Melanoma Manag 7:MMT39

Article  PubMed  PubMed Central  Google Scholar 

Lentini A, Kleinman HK, Mattioli P, Autuori-Pezzoli V, Nicoli L, Pietrini A, Abbruzzese A, Cardinali M, Beninati S (1998) Inhibition of melanoma pulmonary metastasis by methylxanthines due to decreased invasion and proliferation. Melanoma Res 8:131

Article  CAS  PubMed  Google Scholar 

Gude RP, Menon LG, Rao SG (2001) Effect of caffeine, a xanthine derivative, in the inhibition of experimental lung metastasis induced by B16F10 melanoma cells. J Exp Clin Cancer Res 20:287

CAS  PubMed  Google Scholar 

Tabolacci M, Cordella S, Rossi M, Bonaccio A, Eramo C, Mischiati S, Beninati L, Iacoviello AF, Facchiano F (2021) Targeting melanoma-initiating cells by caffeine: in silico and in vitro approaches. Molecules 26:3619

Article  CAS  PubMed  PubMed Central  Google Scholar 

Fagundes TR, Madeira TB, Melo GP, Bordini HP, Marinello PC, Nixdorf SL, Cecchini AL, Luiz RC (2022) Caffeine improves the cytotoxic effect of dacarbazine on B16F10 murine melanoma cells. Bioorganic Chem 120:105576

Article  CAS  Google Scholar 

Burnstock G (1997) The past, present and future of purine nucleotides as signalling molecules. Neuropharmacology 36:1127

Article  CAS  PubMed  Google Scholar 

Young A, Ngiow SF, Barkauskas DS, Sult E, Hay C, Blake SJ, Huang Q, Liu J, Takeda K, Teng MWL, Sachsenmeier K, Smyth MJ (2016) Co-inhibition of CD73 and A2AR adenosine signaling improves anti-tumor immune responses. Cancer Cell 30:391

Article  CAS  PubMed  Google Scholar 

Soleimani A, Farshchi HK, Mirzavi F, Zamani P, Ghaderi A, Amini Y, Khorrami S, Mashayekhi K, Jaafari MR (2020) The therapeutic potential of targeting CD73 and CD73-derived adenosine in melanoma. Biochimie 176:21

Article  CAS  PubMed  Google Scholar 

Bagatini MD, Bertolin K, Bridi A, Pelinson LP, da Silva Rosa B, Bonadiman MM, Pillat PBD, Gonçalves H, Ulrich MRC, Schetinger MR, Morsch VM (2019) 1α, 25-Dihydroxyvitamin D3 alters ectonucleotidase expression and activity in human cutaneous melanoma cells. J of Cell Biochem 120:9992

Article  CAS  Google Scholar 

Jacobson KA, Müller CE (2016) Medicinal chemistry of adenosine, P2Y and P2X receptors. Neuropharmacology 104:31

Article  CAS  PubMed  Google Scholar 

Adinolfi E, Giuliani AL, De Marchi E, Pegoraro A, Orioli E, Di Virgilio F (2018) The P2X7