Biocompatibility assessment of encapsulation materials for micro LED biomedical applications: in vivo and in vitro studies

Greer R, Verdier A, Butt E, Cheng Y, Callas E, McAlinden N, Dawson MD, Bathellier B, Mathieson K. Chronically implantable µLED arrays for optogenetic cortical surface stimulation in mice. bioRxiv. 2025. Preprint: 2025.02.08.637222.

Okui A, Moriya K, Kai N, Yamada M, Nishikawa A, Loesing A, et al. Multipoint MicroLED probes for selective neural stimulation in optogenetics. Jpn J Appl Phys. 2025;64(4):048005. https://doi.org/10.35848/1347-4065/adc863.

Article  Google Scholar 

Novoa RH, Huaman K, Caballero P. Light-emitting diode (LED) phototherapy versus non-LED phototherapy devices for hyperbilirubinemia in neonates: a systematic review and meta-analysis. Am J Perinatol. 2023;40(15):1618–28. https://doi.org/10.1055/a-1827-7607.

Article  Google Scholar 

Zhang H, Peng Y, Zhang N, Yang J, Wang Y, Ding H. Emerging optoelectronic devices based on microscale LEDs and their use as implantable biomedical applications. Micromachines. 2022;13(7):1069. https://doi.org/10.3390/mi13071069.

Article  Google Scholar 

Ban S, Yi H, Park J, Huang Y, Yu KJ, Yeo W-H. Advances in photonic materials and integrated devices for smart and digital healthcare: bridging the gap between materials and systems. Adv Mater. 2025. https://doi.org/10.1002/adma.202416899.

Article  Google Scholar 

Winkler S, Edelmann J, Welsch C, Ruff R. Different encapsulation strategies for implanted electronics: comparison of titanium, ceramic and silicone material for implant body. Curr Dir Biomed Eng. 2017;3(2):725–8. https://doi.org/10.1515/cdbme-2017-0153.

Article  Google Scholar 

Sang M, Kim K, Shin J, Yu KJ. Ultra-thin flexible encapsulating materials for soft bio-integrated electronics. Adv Sci. 2022;9(30):2202980. https://doi.org/10.1002/advs.202202980.

Article  Google Scholar 

Ahn S-H, Jeong J, Kim SJ. Emerging encapsulation technologies for long-term reliability of microfabricated implantable devices. Micromachines. 2019;10(8):508. https://doi.org/10.3390/mi10080508.

Article  Google Scholar 

Mariello M, Kim K, Wu K, Lacour SP, Leterrier Y. Recent advances in encapsulation of flexible bioelectronic implants: materials, technologies, and characterization methods. Adv Mater. 2022;34(34):2201129. https://doi.org/10.1002/adma.202201129.

Article  Google Scholar 

Rogers JA, Ghaffari R, Kim D-H, editors. Stretchable bioelectronics for medical devices and systems. Cham: Springer International Publishing; 2016.

Google Scholar 

Lee S, Kim JS, Wang Y, Tagawa Y, Wang W, Sun L, et al. An ultrasoft nanomesh strain sensor with extreme mechanical durability against friction for on-skin applications. Device. 2025;3(1):100559. https://doi.org/10.1016/j.device.2024.100559.

Article  Google Scholar 

Rosengren A, Faxius L, Tanaka N, Watanabe M, Bjursten LM. Comparison of implantation and cytotoxicity testing for initially toxic biomaterials. J Biomed Mater Res A. 2005;75(1):115–22. https://doi.org/10.1002/jbm.a.30431.

Article  Google Scholar 

Kanďárová H, Pôbiš P. The “Big Three” in biocompatibility testing of medical devices: implementation of alternatives to animal experimentation—Are we there yet? Front Toxicol. 2024. https://doi.org/10.3389/ftox.2023.1337468.

Article  Google Scholar 

Salthouse D, Novakovic K, Hilkens CMU, Ferreira AM. Interplay between biomaterials and the immune system: challenges and opportunities in regenerative medicine. Acta Biomater. 2023;155:1–18. https://doi.org/10.1016/j.actbio.2022.11.003.

Article  Google Scholar 

Lock A, Cornish J, Musson DS. The role of in vitro immune response assessment for biomaterials. J Funct Biomater. 2019;10(3):31. https://doi.org/10.3390/jfb10030031.

Article  Google Scholar 

Miranda I, Souza A, Sousa P, Ribeiro J, Castanheira EMS, Lima R, et al. Properties and applications of PDMS for biomedical engineering: a review. J Funct Biomater. 2022;13(1):2. https://doi.org/10.3390/jfb13010002.

Article  Google Scholar 

Li Y, Xiong Y, Cao W, Zhu Q, Lin Y, Zhang Y, et al. Title not provided. Adv Mater Interfaces. 2021;8:2100872. https://doi.org/10.1002/admi.202100872.

Article  Google Scholar 

Yao S, Zhu Y. Title not provided. Adv Mater. 2015;27:1480–511. https://doi.org/10.1002/adma.201404446.

Article  Google Scholar 

Arakawa ET, Williams MW, Ashley JC, Painter LR. Title not provided. J Appl Phys. 1981;52:3579–82. https://doi.org/10.1063/1.329140.

Article  Google Scholar 

Shu J, Zhou Z, Liang H, Yang X. Polyimide as a biomedical material: advantages and applications. Nanoscale Adv. 2024;6(17):4309–24. https://doi.org/10.1039/D4NA00292J.

Article  Google Scholar 

Hollenberg BA, Richards CD, Richards R, Bahr DF, Rector DM. A MEMS fabricated flexible electrode array for recording surface field potentials. J Neurosci Methods. 2006;153(1):147–53. https://doi.org/10.1016/j.jneumeth.2005.10.016.

Article  Google Scholar 

Malich G, Markovic B, Winder C. Title not provided. Toxicology. 1997;124:179–92. https://doi.org/10.1016/S0300-483X(97)00151-0.

Article  Google Scholar 

Mattes MJ. Title not provided. Br J Cancer. 2007;96:928–36. https://doi.org/10.1038/sj.bjc.6603663.

Article  Google Scholar 

Lindner C, Pröhl A, Abels M, Löffler T, Batinic M, Jung O, et al. Specialized histological and histomorphometrical analytical methods for biocompatibility testing of biomaterials for maxillofacial surgery in (Pre-) clinical studies. In Vivo. 2020;34:3137–52. https://doi.org/10.21873/invivo.12148.

Article  Google Scholar 

Bhat A, Hara TO, Tian F, Singh B. Title not provided. Environ Sci Adv. 2023;2:171–95. https://doi.org/10.1039/D2VA00218C.

Article  Google Scholar 

Costigan A, Hollville E, Martin SJ. Discriminating between apoptosis, necrosis, necroptosis, and ferroptosis by microscopy and flow cytometry. Curr Protoc. 2023;3(12):e951. https://doi.org/10.1002/cpz1.951.

Article  Google Scholar 

Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods. 1983;65(1):55–63. https://doi.org/10.1016/0022-1759(83)90303-4.

Article  Google Scholar 

Regehr KJ, Domenech M, Koepsel JT, Carver KC, Ellison-Zelski SJ, Murphy WL, et al. Biological implications of polydimethylsiloxane-based microfluidic cell culture. Lab Chip. 2009;9(15):2132–9. https://doi.org/10.1039/B903043C.

Article  Google Scholar 

Song K, Han JH, Lim T, Kim N, Shin S, Kim J, et al. Subdermal flexible solar cell arrays for powering medical electronic implants. Adv Healthc Mater. 2016;5(13):1572–80. https://doi.org/10.1002/adhm.201600222.

Article  Google Scholar 

Park G, Chung H-J, Kim K, Lim SA, Kim J, Kim Y-S, et al. Immunologic and tissue biocompatibility of flexible/stretchable electronics and optoelectronics. Adv Healthc Mater. 2014;3(4):515–25. https://doi.org/10.1002/adhm.201300220.

Article  Google Scholar 

Ahmed F, Chorus I, Cotruvo J, Cunliffe D, de Roda Husman AM, Endo T, et al. WHO guidelines for drinking-water quality. 4th ed. Geneva: WHO Press; 2011.

Google Scholar 

National Research Council Subcommittee on Arsenic in Drinking Water. Arsenic in Drinking Water. Washington, DC: National Academies Press; 1999.

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