-Topolin-induced organogenesis in (L.) Pruski fascicular cambium activation and transverse thin cell layers

Arunagiri C, Stanly AM, Shanmugham D (2022) In vitro studies on Singapore daisy (Sphagneticola trilobata) and formulation of dip drink. J Sci Technol 7:322–331. https://doi.org/10.46243/jst.2022.v7.i02.pp322-331

Article  Google Scholar 

Azadi P, Bagheri K, Gholami M, Mirmasoumi M, Moradi A, Sharafi A (2017) Thin cell layer, a suitable explant for in vitro regeneration of saffron (Crocus sativus L.). J Agr Sci Tech 19:1429–1435

Google Scholar 

Bai T, Dong Z, Zheng X, Song S, Jiao J, Wang M, Song C (2020) Auxin and its interaction with ethylene control adventitious root formation and development in apple rootstock. Front Plant Sci 11:574881. https://doi.org/10.3389/fpls.2020.574881

Article  PubMed  PubMed Central  Google Scholar 

Bhattacharyya P, Kumaria S, Job N, Tandon P (2016) En-masse production of elite clones of Dendrobium crepidatum: a threatened, medicinal orchid used in traditional Chinese medicine (TCM). J Appl Res Med Arom Plants 3:168–176. https://doi.org/10.1016/j.jarmap.2016.04.001

Article  Google Scholar 

Borghi SM, Mizokami SS, Carvalho TT, Rasquel-Oliveira FS, Ferraz CR, Fattori V, Hayashida TH, Peron JP, Camilios-Neto D, Ambrosio SR, Arakawa NS, Casagrande R,Verri WA (2021) The diterpene from Sphagneticola trilobata (L.) Pruski, kaurenoic acid, reduces lipopolysaccharide-induced peritonitis and pain in mice. J Ethnopharmacol 273:113980.https://doi.org/10.1016/j.jep.2021.113980

Dobránszki J, Teixeira da Silva JA (2013) In vitro shoot regeneration from transverse thin cell layers of apple leaves in response to various factors. J Hortic Sci Biotechnol 88:60–66

Article  Google Scholar 

Dorani E, Dehghanian Z, Gougerdchi V, Hamedpour-Darabi M (2024) Application of somaclonal variation in crop improvements. In Plant mutagenesis: sustainable landscape planning and natural resources management. Springer. pp 93–109. https://doi.org/10.1007/978-3-031-50729-8_8

Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bul 19:11–15

Google Scholar 

Fagundes TR, Taciane da Silva Bortoleti B, Tomiotto-Pellissier F, Concato VM, Gonçalves MD, Arakawa NS, Miranda-Sapla MM, Panis C, Pavanelli WR (2022) Grandiflorenic acid from Wedelia trilobata plant induces apoptosis and autophagy cell death in breast adenocarcinoma (MCF7), lung carcinoma (A549), and hepatocellular carcinoma (HuH7.5) cells lines. Toxicon 217:112–120. https://doi.org/10.1016/j.toxicon.2022.08.006

Fehér A (2019) Callus, dedifferentiation, totipotency, somatic embryogenesis: what these terms mean in the era of molecular plant biology? Front Plant Sci 10:536. https://doi.org/10.3389/fpls.2019.00536

Article  PubMed  PubMed Central  Google Scholar 

Gao L, Alpert P, Yu H (2020) Parasitism induces negative effects of physiological integration in a clonal plant. New Phytol 229:585–592. https://doi.org/10.1111/nph.16884

Article  CAS  PubMed  Google Scholar 

Gomez KA, Gomez AA (1984) Statistical procedures for agricultural research, 2nd edn. John Wiley and Sons, New York, p 680

Google Scholar 

HEAR (2008) Alien species in Hawaii. Hawaii Ecosystems at Risk. Honolulu, USA: University of Hawaii. http://www.hear.org/AlienSpeciesInHawaii/index.html

Hnatuszko-Konka K, Gerszberg A, Weremczuk-Jeżyna I, Grzegorczyk-Karolak I (2021) Cytokinin signaling and de novo shoot organogenesis. Genes 12:265. https://doi.org/10.3390/genes12020265

Article  CAS  PubMed  PubMed Central  Google Scholar 

Hussain SA, Anis M, Alharby HF, Hakeem KR (2024) In vitro regeneration potential of shoot tip explants, ex vitro rooting and phytochemical assessment of acetone leaf extracts of Tecoma stans L. S Afr J Bot 169:109–118. https://doi.org/10.1016/j.sajb.2024.04.015

Article  CAS  Google Scholar 

Iqbal A, Khan RS, Khan MA, Gul K, Aizaz M, Usman M, Arif M (2023) Efficient regeneration in sugarcane using thin cell layer (TCL) culture system. Sugar Tech 25:168–176. https://doi.org/10.1007/s12355-022-01162-y

Article  CAS  Google Scholar 

Jedrzejczyk I (2020) Genome size and SCoT markers as tools for identification and genetic diversity assessment in Echinacea genus. Ind Crops Prod 144:112055. https://doi.org/10.1016/j.indcrop.2019.112055

Article  CAS  Google Scholar 

Jogam P, Sandhya D, Shekhawat MS, Alok A, Manokari M, Abbagani S, Allini VR (2020) Genetic stability analysis using DNA barcoding and molecular markers and foliar micro-morphological analysis of in vitro regenerated and in vivo grown plants of Artemisia vulgaris L. Ind Crops Prod 151:112476. https://doi.org/10.1016/j.indcrop.2020.112476

Article  CAS  Google Scholar 

Keerthiga M, Anand SP, Nandagopalan V, Doss A, Senthilkumar SR (2012) In vitro propagation of Wedelia trilobata (L.) using Phormidium subincrustatum extracts: a novel approach. AsPac J Mol Biotechnol 20:147–152

Google Scholar 

Koh KS, Ismail MF, Naharudin NS, Gantait S, Sinniah UR (2024) Harnessing the potential of transverse thin cell layer culture for high-frequency micropropagation of Thai ginseng (Kaempferia parviflora Wall. Ex Baker). Ind Crops Prod 213:118375. https://doi.org/10.1016/j.indcrop.2024.118375

Lalthafamkimi L, Bhattacharyya P, Bhau BS, Wann SB, Banik D (2021) Direct organogenesis mediated improvised mass propagation of Pogostemon cablin: a natural reserve of pharmaceutical biomolecules. S Afr J Bot 140:375–384. https://doi.org/10.1016/j.sajb.2020.08.018

Article  CAS  Google Scholar 

Lardon R, Geelen D (2020) Natural variation in plant pluripotency and regeneration. Plants 9:1261. https://doi.org/10.3390/plants9101261

Article  CAS  PubMed  PubMed Central  Google Scholar 

Larskaya I, Gorshkov O, Mokshina N, Trofimova O, Mikshina P, Klepikova A, Gogoleva N, Gorshkova T (2020) Stimulation of adventitious root formation by the oligosaccharin OSRG at the transcriptome level. Plant Signal Behav 15:1703503. https://doi.org/10.1080/15592324.2019.1703503

Article  CAS  PubMed  Google Scholar 

Lo K, Gansau JA, Shih C, Kao C (2022) Shoot development through modified transverse thin cell layer (tTCL) culture of phalaenopsis hybrid protocorms. Hortic 8:206. https://doi.org/10.3390/horticulturae8030206

Article  Google Scholar 

Mahanta M, Gantait S, Mukherjee E, Bhattacharyya S (2023) meta-Topolin-induced mass propagation, acclimatization and cyto-genetic fidelity assessment of gerbera (Gerbera jamesonii Bolus ex Hooker f.). Safr J Bot 153:236–245. https://doi.org/10.1016/j.sajb.2022.11.032

Article  CAS  Google Scholar 

Manokari M, Jayaprakash K, Cokul Raj M, Dey A, Faisal M, Alatar AA, Joshee N, Shekhawat MS (2023) In vitro micro-morphometric growth modulations induced by N6 cytokinins (meta-Topolin and 6-benzylaminopurine) in Ceropegia juncea Roxb. – a rare medicinal climber. S Afr J Bot 157:656–666. https://doi.org/10.1016/j.sajb.2023.04.045

Article  CAS  Google Scholar 

Mardina V, Ilyas S, Halimatussakdiah H, Harmawan T, Tanjung M, Yusof F (2021) Anticancer, antioxidant, and antibacterial activities of the methanolic extract from Sphagneticola trilobata (L.) J. F Pruski leaves. J Adv Pharma Technol Res 12: 222. https://doi.org/10.4103/japtr.JAPTR_131_21

Martin KP, Beena MR, Joseph D (2003) High frequency axillary bud multiplication and ex vitro rooting of Wedelia chinensis (Osbeck) Merr. – a medicinal plant. Indian J Exp Biol 41:262–266

CAS  PubMed  Google Scholar 

Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15:473–497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x

Article  CAS  Google Scholar 

Naaz A, Hussain SA, Anis M, Alatar AA (2019) meta-Topolin improved micropropagation in Syzygium cumini and acclimatization to ex vitro conditions. Biol Plant 63:174–182. https://doi.org/10.32615/bp.2019.020

Nhut DT, Teixeira Da Silva JA, Aswath CR (2003) The importance of the explant on regeneration in thin cell layer technology. In Vitro Cell Dev Biol - Plant 39:266–276. https://doi.org/10.1079/IVP2002408

Nieminen K, Blomster T, Helariutta Y, Mähönen AP (2015) Vascular cambium development. The Arabidopsis Book / American Society of Plant Biologists 13. https://doi.org/10.1199/tab.0177

Article  PubMed Central  Google Scholar 

Nowakowska K, Pacholczak A (2020) Comparison of the effect of meta-Topolin and benzyladenine during Daphne mezereum L. micropropagation. Agronomy 10:1994. https://doi.org/10.3390/agronomy10121994

Ou S, Shi H, Tsang EP (2010) Induction and in vitro culture of Wedelia trilobata hairy roots. Sheng Wu Gong Cheng Xue Bao 26:378–385

CAS  PubMed  Google Scholar 

Pardi R, Basile GN, De Mastro G, Gargano ML, Tagarelli A, Ruta C (2025) Efficient micropropagation by ex vitro rooting of Myrtus communis L. Hortic 11:207. https://doi.org/10.3390/horticulturae11020207

Article  Google Scholar 

Pillitteri LJ, Guo X, Dong J (2016) Asymmetric cell division in plants: mechanisms of symmetry breaking and cell fate determination. Cell Mol Life Sci CMLS 73:4213. https://doi.org/10.1007/s00018-016-2290-2

Article  CAS  PubMed  Google Scholar 

Prasanth DSNBK, Lakshmana RA (2018) Evaluation of pharmacognostic, phytochemical and physicochemical standards of Wedelia trilobata (L.) root. Pharm Res 2:000149. https://doi.org/10.23880/OAJPR-16000149

Qi S, Dai Z, Miao S, Zhai D, Si C, Huang P, Wang R, Du D (2014) Light limitation and litter of an invasive clonal plant, Wedelia trilobata, inhibit its seedling recruitment. Ann Bot 114:425–433. https://doi.org/10.1093/aob/mcu075

Article  PubMed  PubMed Central  Google Scholar 

Rahman MM, Bhadra SK (2011) Development of protocol for in vitro culture and rapid propagation of Wedelia chinensis (Osbeek) Merr. J Med Plants Res 5:2387–2392

CAS  Google Scholar 

Reshi ZA, Husain FM, Khanam MN, Javed SB (2025) Effect of meta-Topolin on morphological, physiochemical, and molecular dynamics during in vitro regeneration of Salix tetrasperma Roxb. BMC Plant Biol 25:121. https://doi.org/10.1186/s12870-025-06095-8

Article  CAS  PubMed  PubMed Central  Google Scholar 

Samarina LS, Malyarovskaya VI, Reim S, Yakushina LG, Koninskaya NG, Klemeshova KV, Shkhalakhova RM, Matskiv AO, Shurkina ES, Gabueva TY, Slepchenko NA, Ryndin AV (2021) Transferability of ISSR, SCoT and SSR markers for Chrysanthemum × Morifoliumramat and genetic relationships among commercial Russian cultivars. Plants 10:1302.

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