Heteroploid plants of Anthurium andreanum 'Tropical Red' were successfully induced by treating diploid callus with colchicine. Callus induction and proliferation were achieved on half-strength MS medium supplemented with 1.0 mg L⁻1 BA and 0.5 mg L⁻1 2,4-D. The resulting callus were exposed to colchicine at three concentrations (0.1%, 0.2%, and 0.3%) for durations of 3, 5, and 7 h. Following treatment, callus were transferred to shoot induction medium, and ploidy levels were determined using flow cytometry. Tetraploid plants were obtained in all treatments with induction rates ranging from 1.20 ± 0.08% to 7.00 ± 0.40%. Increasing colchicine concentration and exposure time significantly reduced explant survival. The optimal treatment was identified as 0.2% colchicine for 7 h, which resulted in 6.40 ± 0.14% tetraploid induction, 7.00 ± 0.14% mixoploid formation, and a 50.00% survival rate following shoot multiplication. These results demonstrate the feasibility of in vitro-induced heteroploidy in A. andreanum and provide a foundation for future breeding and germplasm improvement efforts.