The health risks and environmental safety issues arising from the long-term extensive use of glyphosate have become a global concern. To develop a simple, rapid, and low-cost detection method for the field detection of glyphosate, we successfully constructed a metal-organic framework (MOF-818) with peroxidase (POD)-like activity. Kinetic analysis and catalytic mechanism investigations revealed the critical regulatory role of copper elements in modulating the POD-like activity. By leveraging the principle of glyphosate-induced catalytic inhibition through specific chelation with Cu²⁺ in MOF-818, we developed a "signal-off" detection system for glyphosate residues. Experimental results demonstrated this detection platform exhibited a linear range from 2 μM to 100 μM (R² = 0.99) with a low detection limit (LOD) of 1 μM. And it showed excellent anti-interference performance even in the complex interfering ions. In addition, the relative standard deviations (RSD) of three environmental water samples with varying pollution levels were all below 9%, demonstrating the method reliability in practical applications. This work not only elucidated the pivotal function of metal centers in enzyme-mimetic catalysis, but also provided a reliable technical foundation for developing portable field detection devices, showing significant application potential for real-time monitoring of glyphosate contamination in aquatic.

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