Proteomic analysis of X-linked dystonia parkinsonism disease striatal neurons reveals altered RNA metabolism and splicing

Keywords

Neurodegeneration

X-linked dystonia parkinsonism disease

Induced pluripotent stem cells

Medium spiny neurons

Quantitative proteomics

RNA metabolism

RNA splicing

AbbreviationsATF2

activating transcription factor 2

CNS

central nervous system

DDA

data-dependent acquisition

DIA

data-independent acquisition

EGR2

early growth response 2

GABA

gamma-aminobutyric acid

iPSC

induced pluripotent stem cells

KEGG

Kyoto Encyclopedia of Genes and Genomes

LC-MS/MS

liquid chromatography-tandem mass spectrometry

LGE

lateral ganglionic eminence

MAP2

microtubule associated protein 2

MRPs

mitochondrial ribosomal protein families

PCA

principal component analysis

POLDIP3

DNA polymerase delta interacting protein 3

SCA

spinocerebellar ataxia

SOX2

SRY-box transcription factor 2

SRSFs

serine and arginine rich splicing factor families

TAF1

TATA-binding protein associated factor 1

TAF1-32i

TAF1 aberrant transcript that is composed the canonical exon32 spliced to a cryptic exon in intron 32

TFIID

transcription factor II D

TUBB3

tubulin beta 3 class III

WGCNA

weighted coexpression network analysis

XDP

X-linked dystonia-parkinsonism

XIC

extracted ion chromatogram

YY1

YY1 transcription factor

Data availability

Raw data, complete MS data sets, and spectral libraries have been uploaded to the Center for Computational Mass Spectrometry and to the MassIVE repository at UCSD and can be downloaded using the following link: https://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=f168b6b041ae47a5bbe6ffb63c100b20 (MassIVE ID number: MSV000092344; ProteomeXchange ID: PXD043562).

[Note to the reviewers: To access the data repository MassIVE (UCSD) for MS data, please use: Username: MSV000092344_reviewer; Password: winter].

© 2023 Published by Elsevier Inc.

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