MAPPING MOLECULAR INTERACTION INTERFACE BETWEEN DIAPHANOUS FORMIN-2 AND NEURON-SPECIFIC DREBRIN A

Keywords

actin

drebrin

formin

mass spectrometry

molecular interaction interface

mapping

AbbreviationsDrbA-FL

full length recombinant mouse drebrin A

DrbA-dCT

drebrin A with the C-terminal deletion of aa684-706

Drb1-300

drebrin’s aa1-300

Drb2-252

drebrin’s aa2-252

Drb233-317

drebrin’s aa233-317

P1-P5

partially overlapping synthetic peptides covering aa205-262 of drebrin

CT

synthetic peptide of drebrin corresponding to the C-terminal aa691-706

CT-2A

drebrin CT E703A/D706A

CT-4A

drebrin CT E697A/E698A/E699A/E700A

mDia2-FH2

FH2 domain of mouse diaphanous formin-2 (aa612–1034)

mDia2-FFC

FH1-FH2 domains of mouse diaphanous formin-2 (aa521–1171)

© 2023 The Author(s). Published by Elsevier Ltd.

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