Objectives To evaluate the erosive potential of an energy drink modified with different calcium formulations and concentrations on dental enamel.
Methods This in vitro study used sixty dental enamel specimens, divided into 12 groups: traditional Red Bull® energy drink modified with three concentrations each of calcium/phosphorus/potassium complex (0.71, 1.43, and 2.15g), dicalcium malate (0.83, 1.66, and 2.50g), and calcium citrate malate (1.26, 2.53, and 3.80g); unmodified traditional Red Bull® energy drink; deionized water; and commercially available calcium-supplemented Life Mix Baixa Caloria® orange juice. The pH of all drinks was measured using a pHmeter. Specimens were exposed to the drinks for two minutes. Surface roughness and microhardness were assessed before and after exposure using a rugosimeter and a Vickers microhardness tester, respectively.
Results The addition of calcium/phosphorus/potassium complex increased the energy drink’s pH proportionally to the concentration added. Specimens exposed to modified drinks showed reduced roughness (p < 0.004) compared to control drinks. Drinks modified with 2.50g of dicalcium malate and 2.15g of the calcium/phosphorus/potassium complex significantly preserved enamel microhardness (p < 0.004) compared to the unmodified energy drink. Across all modifications, enamel surface microhardness loss was lower than in control groups, except for deionized water.
Conclusion The addition of calcium formulations to the energy drink effectively reduced its erosive potential at all concentrations, with the calcium/phosphorus/potassium complex demonstrating the greatest protective effect.
Competing Interest StatementThe authors have declared no competing interest.
Funding StatementThe author(s) received no specific funding for this work.
Author DeclarationsI confirm all relevant ethical guidelines have been followed, and any necessary IRB and/or ethics committee approvals have been obtained.
Yes
The details of the IRB/oversight body that provided approval or exemption for the research described are given below:
The study was approved for execution by the Research Ethics Committee of the Universidade do Estado do Rio Grande do Norte on May 15, 2023, under protocol number: 6.059.387.
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Yes
I understand that all clinical trials and any other prospective interventional studies must be registered with an ICMJE-approved registry, such as ClinicalTrials.gov. I confirm that any such study reported in the manuscript has been registered and the trial registration ID is provided (note: if posting a prospective study registered retrospectively, please provide a statement in the trial ID field explaining why the study was not registered in advance).
Yes
I have followed all appropriate research reporting guidelines, such as any relevant EQUATOR Network research reporting checklist(s) and other pertinent material, if applicable.
Yes
Data AvailabilityAll relevant data are within the manuscript and its Supporting Information files.
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