Cyclosporine A (CsA) is an immunosuppressive drug that widely used in clinic. Due to its narrow therapeutic window and the significant individual differences, therapeutic drug monitoring (TDM) of CsA is required to ensure the safety. In this study，we screened a novel aptamer named CsA7 which can specifically recognize CsA, and developed an AuNPs colorimetric aptasensor for the rapid detection of CsA. In the SELEX, after eight rounds of screening, four aptamer candidate sequences were obtained and subjected to the binding affinity and specificity test. Finally, the CsA7 aptamer (Kd=41.21 ng/mL) showed the highest affinity and best specificity for CsA. Based on the CsA7, we also developed an AuNPs colorimetric aptasensor, which had a detection limit of 0.1 ng/mL and a quantitative range of 0.1-500 ng/mL and showed the good selectivity between the CsA and its analogs. According to the results, the CsA7 aptamer provides an alternative recognition molecule to the antibody in biosensor applications and shows great potential for the rapid and convenient detection of CsA.

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