Advances in Pharmacology and Pharmacy Vol. 11(4), pp. 319 - 328
DOI: 10.13189/app.2023.110408
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Thiyagarajan Bharathi , Ganesan Janani , Manokaran Saravanan , Rajangam Udayakumar *
Department of Biochemistry, Government Arts College (Autonomous), Kumbakonam - 612 002 (Affiliated to Bharathidasan University, Tiruchirappalli - 620 024), India

ABSTRACT

Raphanus sativus was selected for this study to analyze phytochemicals, IVAD and IVAI activities. The fresh plant of Raphanus sativus was collected and cleaned with pure water. The aerial part and root peel were separated and then dried under shade. The dried plant samples were ground well into powder. About 100 g of dry powder was extracted with solvents ethanol, methanol and benzene by soxhlet apparatus. The extracts were filtered and kept in oven at 40℃ to evaporate the solvent completely from it. Dark brown residues were obtained and kept in air tight containers separately at 4℃ until the time of use. The aerial part and root peel extracts of Raphanus sativus were used for the evaluation of phytochemicals, IVAD and IVAI activities. The phytochemical screening of extracts revealed the presence of phenolic compounds, flavonoids, alkaloids, etc. The ethanolic, methanolic and benzene extracts of aerial part of Raphanus sativus showed IVAD activity as 75.17 ± 3.60%, 71.70 ± 3.47% and 72.61 ± 3.66%, respectively at 250 μg/ml of concentration. The ethanolic, methanolic and benzene extracts of root peel of Raphanus sativus showed IVAD activity as 78.61 ± 3.62%, 72.73 ± 2.45% and 71.50 ± 3.75%, respectively at 250 μg/ml of concentration. The IVAI activity was observed in ethanolic, methanolic and benzene extracts of root peel of Raphanus sativus as 83.87 ± 3.55%, 61.82 ± 3.47% and 67.87 ± 3.75%, respectively and the aerial part showed as 82.79 ± 3.65%, 53.97 ± 3.43% and 57.95 ± 3.47%, respectively at 200 μg/ml of concentration. All the tested extracts were exhibited IVAD and IVAI activities as like as reference drugs acarbose and diclofenac sodium, respectively. So, the results suggest that the aerial part and root peel of Raphanus sativus possess phytocompounds, IVAD and IVAI activities.

KEYWORDS
Antidiabetic, Anti-Inflammatory, Aerial Part, Root Peel, Raphanus Sativus

Cite This Paper in IEEE or APA Citation Styles
(a). IEEE Format:
[1] Thiyagarajan Bharathi , Ganesan Janani , Manokaran Saravanan , Rajangam Udayakumar , "Phytochemicals, In Vitro Antidiabetic (IVAD) and Anti-inflammatory (IVAI) Studies of Aerial Part and Root Peel of Raphanus sativus L.," Advances in Pharmacology and Pharmacy, Vol. 11, No. 4, pp. 319 - 328, 2023. DOI: 10.13189/app.2023.110408.

(b). APA Format:
Thiyagarajan Bharathi , Ganesan Janani , Manokaran Saravanan , Rajangam Udayakumar (2023). Phytochemicals, In Vitro Antidiabetic (IVAD) and Anti-inflammatory (IVAI) Studies of Aerial Part and Root Peel of Raphanus sativus L.. Advances in Pharmacology and Pharmacy, 11(4), 319 - 328. DOI: 10.13189/app.2023.110408.