Advances in Pharmacology and Pharmacy Vol. 11(4), pp. 311 - 318
DOI: 10.13189/app.2023.110407
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Abhishek Kumar Pandey 1,*, Preeti Pandey 2
1 Department of Botany, Kalinga University, India
2 Department of Chemistry, Kalinga University, India

ABSTRACT

In the present scenario, none of the impactful drugs is available to treat inflammation and oxidative stress without any side effects. We focused on the Ocimum americanum leaf and evaluated its phytochemical composition, anti-inflammatory properties, and antioxidant potential using an ethanolic extraction method. A spectrophotometer was used to measure the leaf extract's overall phenolic content (TPC), whole tannin content (TTC), and the total amount of flavonoid (TFC). The human red blood cell (HRBC) membrane stabilisation method was used to evaluate the extract's anti-inflammatory properties, and the, -diphenyl--picrylhydrazyl (DPPH) method was used to test its antioxidant capacity. We discovered that the TPC, TTC, and TFC values for the plant extract were 131.44 g of GAE/mg, 13.7 g/GAE mg, and 164.7 g of QE/mg, respectively. With a maximal inhibition of 89.79% at a concentration of 1000 g/ml, the extract demonstrated considerable anti-inflammatory efficacy that was comparable to that of the common medicine diclofenac sodium. The extract's capacity to scavenge DPPH was likewise superior to that of the ascorbic acid standard medication, with 96.32% scavenging being seen at the highest concentration (75 g/ml). The extract from the leaf IC50 value was 13.82±2.44 µg/ml, which was one-fourth that of ascorbic acid (51.88±3.24 µg/ml). Our results indicate that the O. americanum leaf extract has the potential to effectively treat inflammation and oxidative stress, and any useful compounds extracted from this plant could be utilized for this purpose in the future.

KEYWORDS
Antioxidant, Anti-inflammatory drug, Herbal Medicine, Phytochemicals

Cite This Paper in IEEE or APA Citation Styles
(a). IEEE Format:
[1] Abhishek Kumar Pandey , Preeti Pandey , "Evaluation of Phytochemical and In Vitro Anti-inflammatory and Antioxidant Potential of Ocimum americanum L. Leaf," Advances in Pharmacology and Pharmacy, Vol. 11, No. 4, pp. 311 - 318, 2023. DOI: 10.13189/app.2023.110407.

(b). APA Format:
Abhishek Kumar Pandey , Preeti Pandey (2023). Evaluation of Phytochemical and In Vitro Anti-inflammatory and Antioxidant Potential of Ocimum americanum L. Leaf. Advances in Pharmacology and Pharmacy, 11(4), 311 - 318. DOI: 10.13189/app.2023.110407.