Development of an improved and simple shoot tip cryoconservation protocol for cryobanking of Bacopa monnieri (L.) Wettst. germplasm

Agrawal A, Sanayaima R, Singh R, Tandon R, Verma S, Tyagi RK (2014) Phenotypic and molecular studies for genetic stability assessment of cryopreserved banana meristems derived from field and in vitro explant sources. In Vitro Cell Dev Biol - Plant 50:345–356. https://doi.org/10.1007/s11627-014-9606-4

Article  CAS  Google Scholar 

Agrawal A, Gowthami R, Chander S, Srivastava V (2022a) Sustainability of in vitro genebanks and cryogenebanks. Indian J Plant Genet Resour 35:180–184. https://doi.org/10.5958/0976-1926.2022.00065.1

Article  Google Scholar 

Agrawal A, Sharma N, Gupta S, Bansal S, Srivastava V, Malhotra EV, Chander S, Gowthami R, Singh K (2022b) Biotechnological applications for plant germplasm conservation at ICAR-National Bureau of Plant Genetic Resources, India–recent achievements. Acta Hort 1339:29–42. https://doi.org/10.17660/ActaHortic.2022.1339.5

Benson EE (2008) Cryopreservation of phytodiversity: A critical appraisal of theory & practice. Crit Rev Plant Sci 27:141–219

Article  CAS  Google Scholar 

Bettoni JC, Bonnart R, Shepherd AN, Kretzschmar AA, Volk GM (2019a) Modifications to a Vitis shoot tip cryopreservation procedure: effect of shoot tip size and use of cryoplates. CryoLetters 40:103–112

PubMed  Google Scholar 

Bettoni JC, Souza JA, Volk GM, Costa DM, da Silva FN, Kretzschmar AA (2019b) Eradication of latent viruses from apple cultivar ‘Monalisa’shoot tips using droplet-vitrification cryotherapy. Sci Hort 250:12–18

Article  Google Scholar 

Bettoni JC, Bonnart R, Volk GM (2021) Challenges in implementing plant shoot tip cryopreservation technologies. Plant Cell Tiss Org Cult 144:21–34. https://doi.org/10.1007/s11240-020-01846-x

Article  Google Scholar 

Decruse SW, Seeni S, Nair GM (2004) Preparative procedures and culture media effect on the success of cryostorage of Holostemma anmulare shoot tips. Plant Cell Tiss Org Cult 76:179–182. https://doi.org/10.1023/B:TICU.0000007283.28280.a3

Article  Google Scholar 

Dixit-Sharma S, Ahuja-Ghosh S, Mandal BB, Srivastava PS (2005) Metabolic stability of plants regenerated from cryopreserved shoot tips of Dioscorea deltoidea – an endangered medicinal plant. Sci Hort 105:513–517. https://doi.org/10.1016/j.scienta.2005.02.011

Article  CAS  Google Scholar 

Doyle JJ, Doyle JJ (1987) A rapid DNA isolation procedure from small quantities of fresh leaf tissues. Phytochem Bull 19:11–15

Google Scholar 

Dubey V (2015) Ecophysiological studies on few threatened medicinal plants of Indore district with special reference to their floral biology. Ph.D. Thesis, Department of Botany, PMB Gujarati College, Devi Ahilaya Vishwavidalaya, Indore, Madhya Pradesh, India. https://hdl.handle.net/10603/127517

Engelmann F (2014) Cryopreservation of clonal crops: a review of key parameters. Acta Hort 1039:31–39. https://doi.org/10.17660/ActaHortic.2014.1039.2

Article  Google Scholar 

González-Arnao MT, Ravelo MM, Urra Villavicencio C, Montero MM, Engelmann F (1998) Cryopreservation of pineapple (Ananas comosus) apices. CryoLetters 19:375–382

Google Scholar 

Gowthami R, Sharma N, Pandey R, Agrawal A (2021) Status and consolidated list of threatened medicinal plants of India. Genet Resour Crop Evol 68:2235–2263. https://doi.org/10.1007/s10722-021-01199-0

Article  CAS  PubMed  PubMed Central  Google Scholar 

Gowthami R, Chander S, Pandey R, Shankar M, Agrawal A (2023) Development of efficient and sustainable droplet-vitrification cryoconservation protocol for shoot tips for long-term conservation of Dahlia germplasm. Sci Hort 321:112329. https://doi.org/10.1016/j.scienta.2023.112329

Article  CAS  Google Scholar 

Harding K, Johnston JW, Benson EE (2009) Exploring the physiological basis of cryopreservation success and failure in clonally propagated in vitro crop plant germplasm. Agric Food Sci 18:103–116. https://doi.org/10.2137/145960609789267524

Article  CAS  Google Scholar 

Jaccard P (1908) Nouvelles Recherches sur la distribution florale. Bull Soc Vaud Sci Nat 44:223–270

Google Scholar 

Jauhari N, Menon S, Sharma N, Bharadvaja N (2017) Uptake of heavy metals from industrial wastewater using in vitro plant cultures. Bull Environ Contam Toxicol 99:614–618. https://doi.org/10.1007/s00128-017-2183-6

Article  CAS  PubMed  Google Scholar 

Jeyasri R, Muthuramalingam P, Suba V, Ramesh M, Chen JT (2020) Bacopa monnieri and their bioactive compounds inferred multi-target treatment strategy for neurological diseases: A cheminformatics and system pharmacology approach. Biomolecules 10:536. https://doi.org/10.3390/biom10040536

Article  CAS  PubMed  PubMed Central  Google Scholar 

Jiang X-R, Ren R-F, Di W, Jia M-X, Li Z-D, Liu Y, Gao R-F (2019) Hydrogen peroxide and nitric oxide are involved in programmed cell death induced by cryopreservation in Dendrobium protocormlike bodies. Plant Cell Tiss Org Cult 137:553–563. https://doi.org/10.1007/s11240-019-01590-x

Article  CAS  Google Scholar 

Kaya E, Souza FVD (2017) Comparison of two PVS2-based procedures for cryopreservation of commercial sugarcane (Saccharum spp.) germplasm and confirmation of genetic stability after cryopreservation using ISSR markers. In Vitro Cell Dev Biol - Plant 53:410–417. https://doi.org/10.1007/s11627-017-9837-2

Article  CAS  Google Scholar 

Mandal BB, Dixit S (2000) Cryopreservation of shoot-tips of Dioscorea deltoidea Wall. – an endangered medicinal yam, for long-term conservation. IPGRI Newslett Asia, Pacific Oceania 33:23

Google Scholar 

Mandal BB, Dixit-Sharma S (2007) Cryopreservation of in vitro shoot tips of Dioscorea deltoidea Wall. – an endangered medicinal plant: effect of cryogenic procedure and storage duration. CryoLetters 28:461–470

Marković Z, Chatelet P, Sylvestre I, Kontić J, Engelmann F (2013) Cryopreservation of grapevine (Vitis vinifera L.) in vitro shoot tips. Open Life Sci 8:993–1000. https://doi.org/10.2478/s11535-013-0223-8

Article  CAS  Google Scholar 

Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x

Article  CAS  Google Scholar 

Muthiah JVL, Shunmugiah KP, Manikandan R (2013) Genetic fidelity assessment of encapsulated in vitro tissues of Bacopa monnieri after 6 months of storage by using ISSR and RAPD markers. Turkish J Bot 37:1008–1017. https://doi.org/10.3906/bot-1207-24

Article  CAS  Google Scholar 

Normah MN, Sulonga N, Reed B (2019) Cryopreservation of shoot tips of recalcitrant and tropical species. Adv Strat Cryobiol 87:1–14. https://doi.org/10.1016/j.cryobiol.2019.01.008

Article  CAS  Google Scholar 

Panis B (2019) Sixty years of plant cryopreservation: from freezing hardy mulberry twigs to establishing reference crop collections for future generations. Acta Hortic 1234:1–8. https://doi.org/10.17660/ActaHortic.2019.1234.1

Article  Google Scholar 

Panis B, Piette B, Swennen R (2005) Droplet vitrification of apical meristems: a cryopreservation protocol applicable to all Musaceae. Plant Sci 168:45–55. https://doi.org/10.1016/j.plantsci.2004.07.022

Article  CAS  Google Scholar 

Panis B, Nagel M, Van den Houwe I (2020) Challenges and prospects for the conservation of crop genetic resources in field genebanks, in in vitro collections and/or in liquid nitrogen. Plants 9:1634. https://doi.org/10.3390/plants9121634

Article  CAS  PubMed  PubMed Central  Google Scholar 

Pathirana R, Mathew L, McLachlan A (2021) A simplified method for high recovery of kiwifruit (Actinidia spp.) shoot tips after droplet vitrification cryopreservation suitable for long-term conservation. Plant Cell Tiss Org Cult 144:97–102. https://doi.org/10.1007/s11240-020-01860-z

Article  CAS  Google Scholar 

Ray A, Bhattacharya S (2008) Cryopreservation of in vitro grown nodal segments of Rauvolfia serpentina by PVS2 vitrification. CryoLetters 29:321–328

PubMed  Google Scholar 

Reed BM (2008) Plant cryopreservation: a practical guide. Springer, New York. https://doi.org/10.1007/978-0-387-72276-4

Book  Google Scholar 

Rohlf FJ (1998) NTSYSpc, numerical taxonomy and multivariate analysis system. version 2.0 user guide. Applied Biostatistics Inc., New York, USA, p 37

Saha PS, Sarkar S, Jeyasri R, Muthuramalingam P, Ramesh M, Jha S (2020) In vitro propagation, phytochemical and neuropharmacological profiles of Bacopa monnieri (L.) Wettst.: A review. Plants 411. https://doi.org/10.3390/plants9040411

Sakai A, Kobayashi S, Oiyama I (1990) Cryopreservation of nucellar cells of navel orange (Citrus sinensis Osb. var. brasiliensis Tanaka) by vitrification. Plant Cell Rep 9:30–33. https://doi.org/10.1007/BF00232130

Article  CAS  PubMed  Google Scholar 

Sant R, Panis B, Taylor M, Tyagi A (2008) Cryopreservation of shoot-tips by droplet vitrification applicable to all taro (Colocasia esculenta var. esculenta) accessions. Plant Cell Tiss Org Cult 92:107–111. https://doi.org/10.1007/s11240-007-9302-8

Article  Google Scholar 

Sharma N, Sharma B (2003) Cryopreservation of shoot tips of Picrorrhiza kurroa Royle ex Benth., an indigenous endangered medicinal plant through vitrification. CryoLetters 24:181–190

PubMed  Google Scholar 

Sharma N, Satsangi R, Pandey R, Vimala Devi S (2007) In vitro clonal propagation and medium term conservation of Brahmi [Bacopa monnieri (L.) Wettst.]. J Plant Biochem Biotech 16:139–144. https://doi.org/10.1007/BF03321990

Article  CAS  Google Scholar 

Sharma N, Satsangi R, Pandey R (2011) Cryopreservation of shoot tips of Bacopa monnieri (L.) Wettst by vitrification technique. Acta Hort 908:283–288. https://doi.org/10.17660/ActaHortic.2011.908.37

Article  CAS  Google Scholar 

Sharma N, Satsangi R, Pandey R, Singh R, Kaushik N, Tyagi RK (2012) In vitro conservation of Bacopa monnieri (L.) using mineral oil. Plant Cell Tiss Org Cult 111:291–301. https://doi.org/10.1007/s11240-012-0194-x

Article  CAS  Google Scholar 

Sharma N, Singh R, Pandey R (2016) In vitro propagation and conservation of Bacopa monnieri L. In: Mohan Jain (Ed) Protocols for In vitro cultures and secondary metabolite Analysis of Medicinal and Aromatic Plants, Second edition, Methods in Molecular Biology, Vol 1391, Springer Science + Buisness Media, New York, 2016, pp 153–171. https://doi.org/10.1007/978-1-4939-3332-7_11.

Sharma N, Singh R, Pandey R, Kaushik N (2017) Genetic and biochemical stability assessment of plants regenerated from cryopreserved shoot tips of a commercially valuable medicinal herb Bacopa monnieri (L.) Wettst. In Vitro Cell Dev Biol - Plant 53:346–351. https://doi.org/10.1007/s11627-017-9826-5

Article  CAS  Google Scholar 

Sharma N, Pandey R, Gowthami R (2020a) In vitro conservation and cryopreservation of threatened medicinal plants of India. In: Rajasekharan PE, Wani SH (eds) Conservation and utilization of threatened medicinal plants. Springer, India, pp 181–228. https://doi.org/10.1007/978-3-030-39793-7_8

Sharma N, Gowthami R, Pandey R, Agrawal A (2020b) Influence of explant types, non-embryogenic synseed and reduced oxygen environment on in vitro conservation of Bacopa monnieri (L.) Wettst. In Vitro Cell Dev Biol - Plant 56:851–856. https://doi.org/10.1007/s11627-020-10092-x

Article  CAS  Google Scholar 

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