aInstitute of Physiology, Brandenburg Medical School Theodor Fontane, Neuruppin

bGeneral and Visceral Surgery Clinic

cUrology and Children Urology Clinic, University Clinics Brandenburg an der Havel

dInstitute of Vegetative Physiology

eInstitute of Neurophysiology, Center of Physiology, University of Cologne, Cologne

fResearch cluster, Molecular Mechanisms of Cardiovascular Diseases

gFaculty of Health Brandenburg, Brandenburg Medical School Theodor Fontane, Neuruppin, Germany

Correspondence to Olaf Grisk, Institute of Physiology, Brandenburg Medical School Theodor Fontane, Fehrbelliner Straße 38, Neuruppin 16816, Germany. Tel: +49 3391 3914713; e-mail: [email protected]

Abbreviations: ANOVA, analysis of variance; ATP, adenosine triphosphate; EGTA, ethylenglycole-bis(aminoethylether)-N,N,N‘,N‘-tetraacetic acid; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; HEPES2-, [4-(2-hydroxyethyl)piperazine-1-yl]ethanesulfonic acid; h-MA, human mesenteric arteries; HPSS, HEPES-buffered physiological salt solution; h-RA, human intrarenal arteries; HRP, horsereddish peroxidase; KPSS, high potassium physiological salt solution; MAPK, mitogen-activated protein kinase; MgATP, magnesium ATP; m-IA, murine interlobar arteries; MLC, myosin light chain; MLCP, myosin light chain phosphatase; m-MA, murine mesenteric arteries; MYPT1, targeting subunit of the myosin light chain phosphatase; PAK1, p21 (RAC1)-activated kinase; PSS, physiological salt solution; RhoA-GEF, rhoA guanine nucleotide exchange factor; ROK, rho kinase; RSK2, p90 ribosomal S6 kinase 2; SDS-PAGE, sodium dodecylsulfate polyacrylamide gel electrophoresis; TBST, Tris-buffered saline solution with Tween20; TCA, trichloracetic acid

Received 15 September, 2022

Revised 31 January, 2023

Accepted 3 March, 2023

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