Document Type : Original paper

Authors

1 Cell Therapy and Regenerative Medicine Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran.

2 Laser Application in Medical Sciences Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

3 Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

4 Proteomics Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

5 Traditional Medicine and Materia Medica Research Center and Department of Traditional Medicine, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

10.22127/rjp.2022.370438.2011

Abstract

Background and objectives: Widespread consuming of onion as a nutrient in the world implies more investigations about useful and harmful aspects of this common supplementary food. Assessment of molecular mechanism of yellow onion extract on cell line Caco-2 was the aim of this study. Methods: Data was extracted from gene expression omnibus (GEO) database. The gene expression profiles of Caco-2 cells in the presence of yellow onion extract versus control cells were analyzed via GOE2R software. The significant differentially expressed genes (DEGs) were assessed via network analysis and the central nodes were enriched via gene ontology. Results: Thirteen central nodes including JUN, ATF3, DUSP1 VEGF, CDKN1A, SNAI1, HSPB1, MCL1, SQSTM1, SREBF1, MAP1LC3B, EZR, and DUSP5 were identified. The related biological terms in five groups were introduced. JUN as a crucial DEG was highlighted. Conclusion: Based on findings, yellow onion extract effects on the wide range of cellular function such as apoptosis, cell proliferation and more other vital functions. The significant role of c-Jun proto-oncogene (among JUN, ATF3, and DUSP1 as hub-bottlenecks) and “c-Jun-ATF3 complex” biological term group as the affected individuals by yellow onion extract were pointed in this study.

Keywords