Identify the genotype of the Dengue 3 virus circulating in Cuba in 2022.

Brazil, Costa Rica and the United States reported the circulation of a new lineage of the Dengue 3 genotype lll, lineage American II.

Four serum samples of DENV-3 collected in 2022 were partially sequenced in a fragment that encodes the envelope gene. Obtained sequences were compared with Cuban sequences from previous years and with others published at GenBank of genotypes I, II and III. Cuban samples of 2022 were classified as genotype III, American lineage II while the previous Cuban isolates were classified as genotype III, American lineage I. The obtained results constitute the basis for future studies using next generation sequencing that will improve our knowledge on this new lineage and its impact on virus transmission and disease severity.