Identification of bacterial protease domains that cleave human IgM
AbbreviationsCH3
Third constant Ig domain of the heavy chain
DIA-MS
Data-independent acquisition mass spectrometry
Fab
Antigen-binding fragment
Fc
Crystallizable fragment
HCD
Higher-energy C-trap dissociation
IdeS
Immunoglobulin G degrading enzyme from Streptococcus pyogenes
IgMDE
Immunoglobulin M degrading enzyme
IMAC
Immobilized metal affinity chromatography
LC-MS/MS
Liquid chromatography coupled to tandem mass spectrometry
PVDF
Polyvinylidene difluoride
SDS
Sodium dodecyl sulfate
PAGE
Polyacrylamide gel electrophoresis
Keywords
IgM protease
Immunoglobulin-degrading enzyme
Immune response
Human IgM
Pathogenic bacteria
IdeS
Data availability
No data was used for the research described in the article.
© 2023 The Author(s). Published by Elsevier Inc.
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